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转Bt基因棉抗棉铃虫鉴定技术及抗性表示方法研究
引用本文:曹美莲,李朋波,刘惠民,张爱莲,姚晋荣. 转Bt基因棉抗棉铃虫鉴定技术及抗性表示方法研究[J]. 棉花学报, 2000, 12(2): 87-90
作者姓名:曹美莲  李朋波  刘惠民  张爱莲  姚晋荣
作者单位:山西省农科院棉花研究所,运城,044000
基金项目:国家“九五”科技攻关项目(96-002-02-10-02)
摘    要:1997~1998年通过接幼虫和即将孵化的卵,进行转Bt基因棉室内鉴定技术及表示方法研究。结果表明,以接即将孵化的卵粒,4d后观测其取食情况最为简便准确,根据试验结果拟定检测分级标准,提出利用抗虫指数计算各抗虫棉群体抗性,依此可以量化地表示转Bt基因棉的抗虫性。

关 键 词:Bt基因棉;棉铃虫;抗虫性;鉴定
文章编号:10002-7807(2000)02-87-04
修稿时间:1999-01-20

Studies on Identification Technique and Classification Method of Bt Transgenic Cotton for Resistanse to Cotton Boilworm (Helicoverpa armigera H.)
CAO Mei-lian,LI Peng-bo,LIU Hui-min,ZHANG Ai-lian,YAO Jin-rong. Studies on Identification Technique and Classification Method of Bt Transgenic Cotton for Resistanse to Cotton Boilworm (Helicoverpa armigera H.)[J]. Cotton Science, 2000, 12(2): 87-90
Authors:CAO Mei-lian  LI Peng-bo  LIU Hui-min  ZHANG Ai-lian  YAO Jin-rong
Affiliation:CAO Mei-lian,LI Peng-bo,LIU Hui-min,ZHANG Ai-lian,YAO Jin-rong;(Cotton Research Institude,Shanxi Academy of Agricultural Sciences, Yuncheng 044000,China)
Abstract:The identification technique and classification method of Bt transgenic cotton for resistance to cotton bollworm(CBW)were studied by putting hatching CBW eggs on cotton leaves indoors. The result showed that it was easier and more accurate to put hatching eggs on leaves and observe its feeding 4 days later. According to the experimental result, the classification standard was made. It was put forword that the population resistance of each Bt transgenic cotton could be caculated by pest-resistant index. In this way, the resistance of Bt transganic cotton to CBW could be completely and quantitatively expressed.
Keywords:Bt-transgenic cotton   cotton bollworm  insect resistance
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