| 胞质内单精子注射制备转基因猪技术的优势与前景 |
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| 引用本文： | 胡骁睿 朱志伟 黄 菁等. 胞质内单精子注射制备转基因猪技术的优势与前景[J]. 安徽农业科学, 2014, 0(8): 2337-2339,2426 |
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| 作者姓名： | 胡骁睿 朱志伟 黄 菁等 |
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| 作者单位： | [1]浙江师范大学,浙江金华321004 [2]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 |
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| 基金项目： | 浙江省畜禽新品种选育重大专项项目（2012C12906-8-1）;浙江省自然科学基金青年基金（LQ12C17002）. |
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| 摘 要： | 综述了20多年来转基因猪的研究概况,探讨了各种转基因技术的特点,分析了胞质内单精子注射制备转基因猪技术的特点和优势,并展望了单精子注射与人工染色体载体技术相结合制备大片段转基因猪的应用前景。
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| 关 键 词： | 转基因 单精注射 人工染色体 猪胚胎 |
The Advantages and Prospect of Producing Transgenic Porcine by Intracytoplasmic Sperm Injection |
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| HU Xiao-rui,PAN Jian-zhi. The Advantages and Prospect of Producing Transgenic Porcine by Intracytoplasmic Sperm Injection[J]. Journal of Anhui Agricultural Sciences, 2014, 0(8): 2337-2339,2426 |
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| Authors: | HU Xiao-rui PAN Jian-zhi |
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| Affiliation: | et al ( Zhejiang Normal University, Jinhua, Zhejiang 321004 ; Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021 ) |
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| Abstract: | The review was about the development of transgenic porcine in the last two decades. And the characteristics of different kinds of transgenic methods are discussed. This paper expounds the advantages of Intracytoplasmic Sperm Injection（ICSI） in producing transgenic porcine, and describes the application of producing large fragments of transgenic pigs by ICSI combined with artificial chromosome vectors technology. |
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| Keywords: | Transfer genes ICSI Artificial chromosome vectors Pig embryo |
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