沾化冬枣茎尖培养快繁技术研究 |
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引用本文: | 尹魁林,张琼,孙承烨.沾化冬枣茎尖培养快繁技术研究[J].安徽农业科学,2014(8):2269-2270,2325. |
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作者姓名: | 尹魁林 张琼 孙承烨 |
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作者单位: | 山东省沾化县冬枣研究所,山东滨州,271000;山东省果树研究所,山东滨州,256803;沾化县林业局,山东滨州,256803 |
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摘 要: | 目的]研究沾化冬枣离体培养的快速繁殖方法。方法]以沾化冬枣茎尖为材料,以MS基本培养基,添加不同浓度IAA和6-BA进行离体培养及再生体系研究,优选最佳培养条件。结果]外植体最适取材时间为4月下旬,接种培养基为MS+2.5 mg/L GA3;最适增殖培养基为:顶芽切段繁殖MS+0.5 mg/L 6-BA+0.25 mg/L IAA,芽丛分割繁殖MS+0.5 mg/L IBA+2 mg/L ZT;最适生根培养基为1/2 MS+1 mg/L NAA+0.5 mg/L IBA;试管苗移栽的最适基质为蛭石加草炭(1∶1,V/V),成活率可达78.8%。结论]该方法优选了沾化冬枣离体培养的快速繁殖方法,为沾化冬枣的种质资源研究提供了可靠依据。
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关 键 词: | 沾化冬枣(Ziziphus jujuba Mill) 茎尖 组织培养 离体快繁 |
Study on Rapid Propagation Technology of Zhanhua Brumal Jujube Stem Tip Culture |
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Institution: | YIN Kui-lin et al ( Winter Jujube Institute of Zhanhua County, Binzhou, Shandong 256803 ) |
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Abstract: | Objective ] In order to explore the method for rapid propagation of Ziziphus jujuba Mill in vitro. Method ] With stem apex of Z. jujuba as material, MS as basic culture medium added with IAA, 6-BA for in vitro culture, the regeneration system was studied. Result ] The most suitable time for explants is in late April, inoculation medium is MS + GA32.5 mg/L; the most suitable medium for bud proliferation: Cutting propagation of the terminal bud is MS + 0. 5 mg/L 6-RA + 0. 25 mg/L iAA, bud segmentation propagation is MS + IBA 0. 5 mg/L + ZT 2 mg/L; the most suitable medium for rooting is 1/2 MS + 1 mg/L NAA +0.5 mg/L IBA;The optimum matrix of planflets is the vermiculite and peat (1: 1, V/V), the survival rate can up to 78.8%. |
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Keywords: | Ziziphus jujuba Mill Stem tip Tissue culture Rapid propagation in vitro |
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