| 几种SDS-PAGE凝胶电泳染色方法的比较 |
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| 引用本文： | 林方养,杨耀东,万婕,谢志皓,马子龙,吴翼. 几种SDS-PAGE凝胶电泳染色方法的比较[J]. 安徽农业科学, 2014, 0(8): 2295-2296 |
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| 作者姓名： | 林方养 杨耀东 万婕 谢志皓 马子龙 吴翼 |
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| 作者单位： | 中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌,571339;江西中医学院科技学院,江西南昌,330025;中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 海南省自然科学基金（312039）. |
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| 摘 要： | [目的]对几种SDS-PAGE凝胶电泳染色方法进行比较。[方法]从凝胶背景颜色、染色时间及灵敏度的角度对6种SDS-PAGE凝胶电泳快速染脱色方法进行比较研究,并对适用于椰子和油棕总蛋白质SDS-PAGE凝胶电泳的实用方法进行总结。[结果]方法 F最快速,条带清晰,染料及试剂都较少,是一种经济、快速、安全、实用的染色方法,整个过程仅需1 h左右。[结论]试验比较了几种SDSPAGE凝胶电泳染色方法,对进一步开展椰子和油棕蛋白质组学研究具有重要意义。
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| 关 键 词： | SDS-PAGE凝胶电泳 染色方法 比较 |
Comparison of Several SDS-PAGE Gel Electrophoresis Staining Methods |
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| LIN Fang-yang,WU Yi. Comparison of Several SDS-PAGE Gel Electrophoresis Staining Methods[J]. Journal of Anhui Agricultural Sciences, 2014, 0(8): 2295-2296 |
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| Authors: | LIN Fang-yang WU Yi |
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| Affiliation: | et al ( Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan 571339) |
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| Abstract: | [ Objective] To compare several SDS-PAGE gel electrophoresis staining methods. [ Method] From perspective of gel background color, staining time and sensitivity, six SDS-PAGE gel electrophoresis staining and destaining methods were compared, a suitable practical method of total protein SDS-PAGE gel electrophoresis for coconut and oil palm was summarized. [ Result ] Method F is the fastest with clear band, less dyes and kit, which is an economy, rapid, safety, practical staining method. [ Conclusion] The study has significance on further research of coconut and oil palm proteomies. |
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| Keywords: | SDS-PAGE gel electrophoresis Staining methods Comparison |
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