Simple Sequence Repeat Markers for Detecting Sources of Tolerance to PTSL Syndrome in Prunus Persica Rootstocks |
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Authors: | Anna V Blenda Gregory L Reighard William V Baird Albert G Abbott |
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Institution: | (1) Department of Horticulture, Poole Agricultural Center, Clemson University, Clemson, SC 29634-0319, USA;(2) Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, Clemson, SC 29634-0318, USA |
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Abstract: | Summary Peach tree short life (PTSL) is a severe and complex disease syndrome in the Southeast of the United States that causes significant
damage to peach orchards and affects more than 70% of the peach acreage in the region. The ring nematode is known to be one
of the major factors strongly associated with the PTSL syndrome. A peach rootstock (Guardian? Brand BY520-9, also referred to as Guardian? or BY520-9), which is tolerant to ring nematode and PTSL, was released in 1994. To investigate the genetic relatedness of
PTSL tolerant genotypes and identify possible parents of Guardian? selections as sources of the PTSL tolerance trait, 98 genotypes derived from the BY520-9 pedigree, and others showing different
degrees of field resistance or susceptibility to PTSL, were selected for simple sequence repeat analysis. Nineteen of 32 microsatellite
loci were polymorphic. Allele number averaged 2.7 per locus with heterozygosity levels ranging from 0.07 to 0.60. Cluster
analysis of these genotypes produced a dendrogram in agreement with their putative pedigrees. Thirty-eight F4 genotypes from Guardian pedigree were used for the parental identification analyses. Of those, nine most likely parental
genotypes were identified for 10 field tested F5 genotypes produced from open-pollinated selections of BY520-9. |
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Keywords: | neighbor-joining cluster analysis pair-wise marker distances parental identification analysis Mesocriconema xenoplax Guardian? |
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