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| 橡胶树炭疽病菌rDNA-ITS区序列分析 | |
| 引用本文： | 崔昌华,郑肖兰,刘洪平,郑服丛.橡胶树炭疽病菌rDNA-ITS区序列分析[J].热带作物学报,2006,27(4):41-45. |
| 作者姓名： | 崔昌华 郑肖兰 刘洪平 郑服丛 |
| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737;华南热带农业大学环境与植物保护学院,海南,儋州,571737 2. 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737 3. 华南热带农业大学环境与植物保护学院,海南,儋州,571737 |
| 基金项目： | 海南省自然科学基金指导性计划项目(80502)资助 |
| 摘 要： | 应用核糖体rDNA-ITS区的序列分析研究18个不同地区来源的橡胶树炭疽病病原菌。结果表明:测试菌株的ITS扩增区(ITS3-ITS4)约为300bp,无太大的长度变异。ITS核酸序列聚类分析的结果表明:18个测试菌株被聚为2个类群,群与群之间差异不明显,2组之间同源率大约为99.3%,通过在NCBI网站的blast比对分析,结果显示2组均为胶孢炭疽菌(Colletrichum gloeosporioides)。 |
| 关 键 词： | 橡胶树炭疽病菌 rDNA-ITS 序列分析 |
| 收稿时间： | 2005-12-15 |
| 修稿时间： | 2006-07-03 |
Sequence Analysis of ITS Region of rDNA of Colletotrichum gloeosporioides Penz. Sacc |
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| Cui Changhua,Zheng Xiaolan,Liu Hongping,Zheng Fucong.Sequence Analysis of ITS Region of rDNA of Colletotrichum gloeosporioides Penz. Sacc[J].Chinese Journal of Tropical Crops,2006,27(4):41-45. | |
| Authors: | Cui Changhua Zheng Xiaolan Liu Hongping Zheng Fucong |
| Institution: | 1. Environment and Plant Protection Research Institute, CATAS Danzhou Hainan 571737 ;2. College of Environment and Plant Protection, SCUTA Danzhou Hainan 571737 China |
| Abstract: | Genetic divergence among 18 strains belonging to the Colletotrichum gloeosporioides Penz.Sacc was assessed by sequence analysis on the basis of rDNA-ITS region. The PCR amplified ITS region (ITS3-ITS4) was about 300 bp in length in all the tested strains. DNAStar TreeView analysis of congenetic rate band in restriction patterns showed that 18 isolates could be divided into 2 groups. These two groups were found not evidently different from each other with a homology of about 99.3%. Blast comparison through the NCBI web showed these two groups were all Colletotrichum gloeosporioides. |
| Keywords: | Colletotrichum gloeosporioides Penz Sacc rDNA-ITS sequence analysis |
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