| 团头鲂肠黏膜益生乳酸菌的分离及体外抗逆性研究 |
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| 引用本文： | 张洪波,单晓枫,钱爱东,韩云. 团头鲂肠黏膜益生乳酸菌的分离及体外抗逆性研究[J]. 水产科学, 2009, 28(7) |
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| 作者姓名： | 张洪波 单晓枫 钱爱东 韩云 |
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| 作者单位： | 吉林农业大学,动物科技学院,吉林,长春,130118;通榆县瞻榆镇畜禽防疫服务中心,吉林,白城,137200 |
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| 摘 要： | 从团头鲂肠黏膜上分离筛选出2株对嗜水气单胞菌有较强的抑制作用的乳酸菌菌株JK-1和JK-2,通过耐受胆盐、耐受pH值、耐受蛋白酶和耐高温等试验对这2株乳酸菌进行体外抗逆性试验研究.其中在胆盐耐受性试验中,JK-1和JK-2在胆盐浓度0.4%、培养4 h时仍有35%以上的存活率;在pH值耐受性试验时,2株乳酸菌均可在一定的酸性和碱性条件下存活;胰蛋白酶对2株菌的存活率没有影响;在70 ℃的高温中,2株乳酸菌均有一定的耐受性.
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| 关 键 词： | 团头鲂 乳酸菌 分离 抗逆性 |
Isolation and Resistance of Lactobacillus from Intestinal Mucosa of Bluntnose Black Bream(Megalobrama amblycephala) |
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| ZHANG Hong-bo,SHAN Xiao-feng,QIAN Ai-dong,HAN Yun. Isolation and Resistance of Lactobacillus from Intestinal Mucosa of Bluntnose Black Bream(Megalobrama amblycephala)[J]. Fisheries Science, 2009, 28(7) |
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| Authors: | ZHANG Hong-bo SHAN Xiao-feng QIAN Ai-dong HAN Yun |
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| Affiliation: | ZHANG Hong-bo 1,SHAN Xiao-feng 1,QIAN Ai-dong 1,HAN Yun 2 (1.College of Animal Science , Technology,Jilin Agricultural University,Changchun 130118,China,2.Zhanyu Center of Epidemic Prevention,Tongyu,Baicheng 137200,China) |
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| Abstract: | Two strains of Lactobacillus JK-1 and JK-2 were isolated from intestinal mucosa of bluntnose black bream(Megalobrama amblycephala)which showed an effective inhibition to Aeromonas hydrophila.A tolerance test was conducted for cholate,pH,protease,and high temperature.Among the cholate-tolerance test,over 35% of JK-1 and JK-2 survived after culturing for 4 and 0.4% concentration in cholate.And in pH-tolerance test,two strains of lactic acid bacteria survived in both acid and alkaline environment.Trypsin did n... |
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| Keywords: | buntsnout bream Lactobacillus isolation adversity resistance |
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