Mapping of EST-derived CAPS markers in Italian ryegrass (Lolium multiflorum Lam.) |
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| Authors: | Y. Miura M. Hirata M. Fujimori |
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| Affiliation: | Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association, 388-5 Higashiakada, Nasushiobara, Tochigi 329-2742, Japan, E-mail:;;National Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nasushiobara, Tochigi 329-2793, Japan;;Present address: Masahiro Fujimori, Yamanashi Prefectural Dairy Experiment Station, 621-2 Nagasaka-Kamijo, Nagasaka, Hokuto, Yamanashi 408-0021, Japan |
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| Abstract: | New molecular markers derived from expressed sequence tag (EST) sequences were mapped on linkage maps of Italian ryegrass by a two-way pseudo-testcross strategy. cDNA sequences were obtained from various tissues of Italian ryegrass ( Lolium multiflorum ) and converted into cleaved amplified polymorphic sequence (CAPS) markers. Of 260 EST primer pairs that amplified a single band, 74 generated bands that showed clear polymorphisms among individuals of an F1 mapping family. Of the 74 polymorphic marker loci, 69 were mapped on an Italian ryegrass linkage map previously constructed using amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), and simple sequence repeat (SSR) markers. The newly-developed EST-CAPS markers would be useful as an efficient tool to identify genetic markers and to identify candidate genes for quantitative trait loci (QTLs) associated with important traits in Italian ryegrass. |
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| Keywords: | Lolium multiflorum expressed sequence tag cleaved amplified polymorphic sequence linkage map |
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