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Expression of a pea disease resistance response gene in the potato cultivar Shepody |
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| Authors: | Ming-Mei Chang Chin C. Chiang Mark W. Martin Lee A. Hadwiger |
| Affiliation: | 1. Department of Plant Pathology, Washington State University, 99164-6430, Pullman, WA 2. Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, PCTB714, 725 N. Wolfe, 21205, Baltimore, MD 3. USDA-ARS, Washington Sate University Irrigated Agriculture Research and Extension Center, 99350-9687, Prosser, WA |
| Abstract: | The coding sequence from the pea disease resistance response gene 49 (DRR49 gene), was transferred into the potato (Solanum tuberosum L.) cultivar Shepody viaAgrobacterium co-cultivation. Potato leaf explants were co-cultivated withAgrobacterium tumefaciens harboring a binary vector containing the coding sequence from the DRR49 gene under the control of the 35S promoter from cauliflower mosaic virus (CaMV). Transformed potato plants were selected by their resistance to kanamycin. The insertion of foreign DNAs was detected by polymerase chain reaction (PCR) and Southern blot analysis. The expression of DRR49 coding sequence was confirmed via the detection of the corresponding mRNA in northern blot analysis. Field pathogenicity tests indicated that transgenic plants expressing the pea DRR49 mRNA have higher tuber yields than the control plants when grown in PED (potato early dying) infested soil. |
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