Detection of potato leafroll virus by visual inspection,direct tissue blotting and ELISA techniques |
| |
| Authors: | J L Whitworth R G Samson T C Allen A R Mosley |
| |
| Institution: | 1. Dept. of Crop and Soil Science, Oregon State Univ., 97331-3002, Corvallis, OR
2. Dept. of Botany and Plant Pathology, Oregon State Univ., 97331-2902, Corvallis, OR
|
| |
| Abstract: | Greenhouse-grown Russet Burbank and Russet Norkotah plants were tested for potato leafroll virus (PLRV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Visibly infected and healthy plants were obtained from excised eyes of tubers submitted for winter certification tests. Each plant was sectioned into leaf, petiole, stem, root, seed piece, and tuber portions. Tissues were blotted on a nitrocellulose membrane for DTBA and then homogenized for use in a double-antibody sandwich ELISA system. Agreement between the two serological detection methods and with visual readings was high for petioles and stems, but lower for leaf, tuber and root tissues. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting PLRV in stems and petioles |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
