Molecular mapping of cereal cyst nematode resistance in <Emphasis Type="Italic">Triticum monococcum</Emphasis> L. and its transfer to the genetic background of cultivated wheat |
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Authors: | Kuldeep Singh Parveen Chhuneja Inderjit Singh S K Sharma Tosh Garg Monika Garg Beat Keller Harcharan S Dhaliwal |
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Institution: | (1) School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India;(2) Department of Plant Pathology, Punjab Agricultural University, Ludhiana, 141 004, India;(3) Institute of Plant Biology, University of Zurich, Zurich, Switzerland;(4) Present address: Department of Biotechnology, IIT Roorkee, Roorkee, Uttarakhand, India;(5) Present address: International Center for Agricultural Research in Dry Areas (ICARDA), Allepo, Syria |
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Abstract: | Triticum monococcum, the diploid A genome species, harbours enormous variability for resistance to biotic stresses. A spring type T. monococcum acc. 14087 was found to be resistant to Heterodera avenae (cereal cyst nematode, CCN). A recombinant inbred line population (RIL) developed by crossing this accession with a CCN susceptible
T. boeoticum acc. 5088 was used for studying the inheritance and map location of the CCN resistance. Based on composite interval mapping
two QTL, one each on chromosome 1AS and 2AS, were detected. The QTL on 1A, designated as Qcre.pau-1A, appeared to be a major gene with 26% contribution to the overall phenotypic variance whereas the QTL on 2A designated as
Qcre.pau-2A contributed 13% to total phenotypic variation. Qcre.pau-1A is novel, being the only CCN resistance gene mapped in any ‘A’ genome species and none of the other known genes have been
mapped on chromosome 1A. The QTL Qcre.pau-2A might be allelic to Cre5, a CCN resistance gene transferred from Ae. ventricosa and mapped on 2AS. The Qcre.pau-1A was transferred to cultivated wheat using T. durum cv. PBW114 as the bridging species. Selected CCN resistant F8 lines showed introgression for the molecular markers identified to be linked with CCN resistance locus Qcre.pau-1A. Thus, this gene alone could impart complete resistance against CCN. These introgression lines can be used for marker-assisted
transfer of Qcre.pau-1A to elite wheat cultivars. |
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