| 透明颤菌血红蛋白基因对苏氨酸发酵生产的影响 |
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| 引用本文： | 井庆川,武彬,石天虹,刘雪兰,魏祥法,阎佩佩,刘瑞亭.透明颤菌血红蛋白基因对苏氨酸发酵生产的影响[J].山东农业科学,2011(4):96-99. |
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| 作者姓名： | 井庆川 武彬 石天虹 刘雪兰 魏祥法 阎佩佩 刘瑞亭 |
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| 作者单位： | 山东省农业科学院家禽研究所,山东济南,250023 |
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| 基金项目： | 山东省农业科学院青年基金项目“高产苏氨酸基因工程菌的构建及产酸条件优化”(2007YQN026)资助 |
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| 摘 要： | 将透明颤菌血红蛋白（VHb）基因转化至苏氨酸发酵基因工程株E.coli中,研究了导入VHb基因对苏氨酸发酵生产的影响。结果表明,改造后苏氨酸发酵菌对营养物质的利用效率增加。以不同碳源发酵时,摇床培养提高苏氨酸产量28.7%～55.4%,以不同氮源发酵时提高产酸7.4%～36.1%。导入VHb基因对菌体生长动力学的影响较小,但对苏氨酸产酸动力学曲线作用较显著。基因改造提高了菌株的生长与苏氨酸合成能力,并能缓解摇床转速较低时供氧对发酵的限制。
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| 关 键 词： | 透明颤菌 血红蛋白 苏氨酸发酵 |
Effect of Vitreoscilla Hemoglobin Gene Expression on L-threonine Production |
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| JING Qing-chuan,WU Bin,SHI Tian-hong,LIU Xue-lan,WEI Xiang-fa,YAN Pei-pei,LIU Rui-ting.Effect of Vitreoscilla Hemoglobin Gene Expression on L-threonine Production[J].Shandong Agricultural Sciences,2011(4):96-99. |
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| Authors: | JING Qing-chuan WU Bin SHI Tian-hong LIU Xue-lan WEI Xiang-fa YAN Pei-pei LIU Rui-ting |
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| Institution: | JING Qing-chuan,WU Bin,SHI Tian-hong,LIU Xue-lan,WEI Xiang-fa,YAN Pei-pei,LIU Rui-ting(Institute of Poultry Science,Shandong Academy of Agricultural Sciences,Jinan 250023,China) |
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| Abstract: | Vitreoscilla hemoglobin(VHb) gene was expressed in E.coli to study the effect on L-threonine production.The results showed that the expression of VHb gene enhanced the use efficiency of fermentation strain to nutritments.It made the L-threonine production increase by 28.7%～55.4% with different carbon resources and 7.4%～36.1% with different nitrogen resources by shaking culture.It had little influence on cell growth kinetics,but had obvious influence on L-threonine producing kinetics curves.The expression of VHb gene promoted the strain growth and L-threanine production and could reduce the restriction of oxygen supply on fermentation at low shaking speed. |
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| Keywords: | Vitreoscilla Hemoglobin Threonine fermentation |
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