| 棉花胞质不育恢复系2152R恢复基因分子标记的筛选 |
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| 引用本文： | 韩宗福,赵逢涛,李汝忠,王景会,申贵芳,王宗文,陈敏. 棉花胞质不育恢复系2152R恢复基因分子标记的筛选[J]. 山东农业科学, 2011, 0(4): 5-8 |
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| 作者姓名： | 韩宗福 赵逢涛 李汝忠 王景会 申贵芳 王宗文 陈敏 |
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| 作者单位： | 1. 山东棉花研究中心,山东济南,250100
2. 济南大学,山东济南,250022 |
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| 基金项目： | 国家转基因生物重大专项(2008ZX08005-001); 山东省农业良种工程项目(2009LZ00503)资助 |
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| 摘 要： | 以棉花细胞质雄性不育系547A与恢复系2152R杂交获得的后代F2分离群体的293个单株为试材,进行遗传分析并利用SSR、STS标记技术筛选与育性恢复基因紧密连锁的分子标记.田间调查结果显示,恢复基因在F2群体的分离比例符合3∶1,证明恢复基因为一对显性基因.同时,共获得4个SSR标记和1个STS标记与恢复基因连锁,这...
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| 关 键 词： | 棉花 细胞质不育系547A 恢复基因 SSR标记 STS标记 |
Molecular Markers for Restorer Gene of Cytoplasmic Cotton Male Sterile Restorer Line 2152R |
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| HAN Zong-fu,ZHAO Feng-tao,LI Ru-zhong,WANG Jing-hui,SHEN Gui-fang,WANG Zong-wen,CHEN Min. Molecular Markers for Restorer Gene of Cytoplasmic Cotton Male Sterile Restorer Line 2152R[J]. Shandong Agricultural Sciences, 2011, 0(4): 5-8 |
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| Authors: | HAN Zong-fu ZHAO Feng-tao LI Ru-zhong WANG Jing-hui SHEN Gui-fang WANG Zong-wen CHEN Min |
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| Affiliation: | HAN Zong-fu1,ZHAO Feng-tao1,LI Ru-zhong1,WANG Jing-hui1,SHEN Gui-fang1,WANG Zong-wen1,CHEN Min2(1.Shandong Cotton Research Centre,Jinan 250100,China,2.University of Jinan,Jinan 250022,China) |
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| Abstract: | With 293 individuals of F2 segregation population from the crossing of CMS line 547A with restorer line 2125R as materials,the molecular markers linked to the fertility restorer gene were identified by SSR and STS techniques.The filed survey results showed that the segregation ratio of restorer gene was 3∶1,which confirmed that the fertility restorer genes were a pair of dominant genes.Additionally,4 SSR and 1 STS markers were identified to be closely linked to the restorer gene.This laid a foundation for breeding the strong heterosis restorer lines by molecular markers. |
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| Keywords: | Cotton Cytoplasmic male sterile line 547A Restorer gene SSR marker STS marker |
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