| 转果聚糖合成关键酶基因多年生黑麦草的获得及抗旱性的提高 |
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| 引用本文： | 张小芸,何近刚,孙学舞,吴金霞.转果聚糖合成关键酶基因多年生黑麦草的获得及抗旱性的提高[J].草业学报,2011,20(1):111-118. |
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| 作者姓名： | 张小芸 何近刚 孙学舞 吴金霞 |
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| 作者单位： | 中国农业科学院生物技术研究所,北京,100081;中国农业科学院生物技术研究所,北京,100081;河北省农林科学院遗传生理研究所,河北,石家庄,050051 |
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| 基金项目： | 国家973计划项目，863 |
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| 摘 要： | 以多年生黑麦草（品种卡特）胚性愈伤组织为转化受体，利用农杆菌介导的遗传转化方法将冰草果聚糖：果聚糖-１-果糖基转移酶基因(Ac1-FFT)导入黑麦草中，对再生植株喷洒ｂａｓｔａ溶液和ＰＣＲ 法检测，共获得１８个阳性株系，ＲＴ－ＰＣＲ 结果表明，该基因在转基因黑麦草中正常表达。转基因黑麦草株系中的可溶性总糖含量和果聚糖含量明显高于对照植株，耐旱性提高，干旱胁迫６ｄ时其相对含水量和叶绿素含量明显高于对照植株，且下降速度慢，但其电解质渗漏率和丙二醛含量显著低于对照植株，复水后很快复原，而对照植株无法恢复，说明转基因植株中由于干旱处理发生的损伤是可逆的，而对照植株中的损伤是非可逆的。以上结果表明，转基因黑麦草中犃Ac1-FFT的表达及果聚糖合成可能是其耐旱性提高的最重要原因。
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| 关 键 词： | 多年生黑麦草 冰草 果聚糖 果聚糖-1-果糖基转移酶基因 抗旱性 |
| 收稿时间： | 1900-01-01; |
Transformation of Lolium perenne with a fructan:fructan 1-fructosyltransferase gene from Agropyron cristatum and enhancement of drought tolerance in transgenic plants |
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| ZHANG Xiao-yun,HE Jin-gang,SUN Xue-hui,WU Jin-xia.Transformation of Lolium perenne with a fructan:fructan 1-fructosyltransferase gene from Agropyron cristatum and enhancement of drought tolerance in transgenic plants[J].Acta Prataculturae Sinica,2011,20(1):111-118. |
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| Authors: | ZHANG Xiao-yun HE Jin-gang SUN Xue-hui WU Jin-xia |
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| Institution: | ZHANG Xiao-yun1,HE Jin-gang1,2,SUN Xue-hui1,WU Jin-xia1(1.Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China,2.Institute of Genetics and Physiology,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051,China) |
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| Abstract: | Ac1-FFT(fructan:fructan 1-fructosyltransferase) cloned from Agropyron cristatum was transformed into embryogenic callus lines of Lolium perenne using an Agrobacterium mediated method.Transgenic plants were analyzed by basta spraying and PCR amplification.In total,18 independent transgenic lines were obtained with significantly improved drought tolerance.RT-PCR analysis showed that Ac1-FFT expressed strongly in transgenic L.perenne plants but not in control plants.Water soluble carbohydrate and fructan conte... |
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| Keywords: | Lolium perenne Agropyron cristatum fructan:fructan 1-fructosyltransferase fructan drought tolerance |
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