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| 薄皮甜瓜自交系高效组织培养技术的研究 | |
| 引用本文： | 侯丽霞,何启伟,赵双宜,王崇启,董玉梅.薄皮甜瓜自交系高效组织培养技术的研究[J].山东农业科学,2006(3):7-10. |
| 作者姓名： | 侯丽霞 何启伟 赵双宜 王崇启 董玉梅 |
| 作者单位： | 1. 山东农业大学园艺科学与工程学院,山东,泰安,271018;山东省农业科学院蔬菜研究所,山东,济南,250100 2. 山东省农业科学院蔬菜研究所,山东,济南,250100 3. 山东大学生命科学院,山东,济南,250100 |
| 摘 要： | 以薄皮甜瓜自交系为试材,探讨了不同部位外植体、苗龄、激素组合等因素对植株离体再生的影响。其结果是以4~6日龄的无菌苗的子叶柄、下胚轴上端外植体适于不定芽的较高频率分化;两种外植体分化存在明显极性现象,近生长点处为感受外源激素的敏感部位;MS、6-BA 1.5~2.5 mg/L、ZT 1.0~3.0mg/L、NAA 0.1~0.2 mg/L之间配比适于不定芽的分化,分化率达60%~70%;MS+KT 1.5~2.5 mg/L+GA 2.0 mg/L利于丛生芽的伸长生长;高效生根培养基为MS+IBA 1.0 mg/L。 |
| 关 键 词： | 薄皮甜瓜 自交系 组织培养 |
| 文章编号： | 1001-4942（2006）03-0007-04 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-02-21 |
Study on Efficacious Tissue Culture Techniques of Thin-skin Muskmelon Inbred Line |
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| HOU Li-xia,HE Qi-wei,ZHAO Shuang-yi,WANG Chong-qi,DONG Yu-mei.Study on Efficacious Tissue Culture Techniques of Thin-skin Muskmelon Inbred Line[J].Shandong Agricultural Sciences,2006(3):7-10. | |
| Authors: | HOU Li-xia HE Qi-wei ZHAO Shuang-yi WANG Chong-qi DONG Yu-mei |
| Institution: | 1. Horticulture College of Shandong Agricultural University, Taian 271018, China; 2. Vegetable Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3. Life Science College of Shandong University, Jinan 250100, China |
| Abstract: | The thin-skin muskmelon inbred lines were used to study the effects of explants,seedling age,and hormones on plant in vitro regeneration.The results showed that the cotyledon petiole and hypocotyls of 4～6 days seedlings had higher differentiation frequency of adventitious buds.And polarity occurred obviously during explants differentiation,the sensitive regions were near the growing point.The MS media with 6-BA 1.5～2.5 mg/L,ZT 1.0～3.0 mg/L,NAA 0.1～0.2 mg/L was suitable for differentiation of adventitious buds,with the differentiation rate 60%～70%.And the better enlongation media was MS+KT 1.5～2.5 mg/L+GA 2.0 mg/L while the high efficiency rooting media was MS+IBA 1.0 mg/L. |
| Keywords: | Thin-skin muskmelon Inbred line Tissue culture |
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