Three-dimensional readout of flash x-ray images of living sperm in water by atomic-force microscopy |
| |
Authors: | T Tomie H Shimizu T Majima M Yamada T Kanayama H Kondo M Yano M Ono |
| |
Institution: | Electrotechnical Laboratory, Ibaraki, Japan. |
| |
Abstract: | The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subnanosecond corrected] x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height. |
| |
Keywords: | |
|