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Biological and molecular characterization of the response of tomato plants treated with Trichoderma koningiopsis
Authors:CA Moreno  F Castillo  A González  D Bernal  Y Jaimes  M Chaparro  C González  F Rodriguez  S Restrepo  AM Cotes
Institution:1. Centro de Biotecnología y Bioindustria – Corpoica, Km 14 vía Occidente Bogotá-Mosquera, Colombia;3. Centro Internacional de Agricultura Tropical, Unidad de Biotecnología, Recta Cali-Palmira Km 17, Cali, Colombia;1. Gottfried Wilhelm Leibniz Universität Hannover, Institute of Plant Diseases and Plant Protection, Herrenhäuser Straße 2, D-30419 Hannover, Germany;2. Centre for Tropical and Subtropical Agriculture and Forestry (CeTSAF) – Tropenzentrum Georg-August Universität Goettingen Buesgenweg, D-37077, Germany;3. Gottfried Wilhelm Leibniz Universität Hannover, Institute of Technical Chemistry, Schneiderberg, 30419 Hannover, Germany;1. Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung, Taiwan;2. Department of Horticulture, National Chung Hsing University, Taichung, Taiwan;3. The MS Program for Plant Medical Science and Safe Agriculture, National Chung Hsing University, Taichung, Taiwan;1. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China;2. School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China;1. Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, Iran;2. Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, P.O. Box 800.56, 3508 TB Utrecht, The Netherlands;3. Department of Plant Science, Faculty of Agriculture, University of Jiroft, P.O. Box 364, Jiroft, Iran;1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India;2. Bio-Protection Research Centre, Lincoln University, Lincoln, New Zealand;3. Faculty of Biology, The Technion-Israel Institute of Technology, Haifa 32000, Israel;4. Spanish-Portuguese Institute for Agricultural Research (CIALE), Department of Microbiology and Genetics, University of Salamanca, Salamanca, Spain
Abstract:Tomato-Fusarium oxysporum f.sp. radicis-lycopersici pathosystem was used to study induced systemic resistance elicited by Trichoderma koningiopsis (Th003) using the split root model. The ability of the antagonist to promote plant growth was also established. Stem colonization by the pathogen was significantly reduced in treated plants. The induction of resistance was enhanced 6 days after elicitation and when the antagonist was used in a concentration of 105 conidia per ml. Th003 application in seed priming and nursery significantly stimulated plant growth. Gene expression induced by Th003 was evaluated using the tomato TOM1 microarray. Plant treatment with T. koningiopsis affected mRNA levels of 45 genes: 41 in roots and 4 in leaves. Of particular interest was the induction of genes involved in the jasmonic and ethylene transduction pathways found in the microarray analysis and qRT-PCR, which suggest a temporary increment of defense related gene expression response to T. koningiopsis Th003.
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