Effect of jasmonic acid on the induction of polyphenoloxidase and peroxidase activities in relation to date palm resistance against Fusarium oxysporum f. sp. albedinis |
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| Authors: | Fatima Jaiti Jean Luc Verdeil Ismail El Hadrami |
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| Institution: | 1. Laboratoire de Biotechnologies, Protection et Valorisation des Ressources Végétales, Département de Biologie, Faculté des Sciences Semlalia, BP. 2390, 40 000 Marrakech, Morocco;2. Laboratoire d''Histocytologie, UMR 1098, Cirad-amis, Cirad, Avenue Agropolis, 34398 Montpellier, cedex 5, France;1. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium;2. Plant-Crop Protection, Institute for Agricultural and Fisheries Research – ILVO, Burg. Van Gansberghelaan 96, B-9820 Merelbeke, Belgium;3. BCCM/LMG Bacteria Collection – Laboratory of Microbiology Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium;1. The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan;2. Botany Department Faculty of Science, Aswan University, Aswan 81528, Egypt;3. Plant Healthcare and Diagnostic Center, PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad 580 003, Karnataka, India;4. Laboratory of Molecular Plant Pathology, Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan;5. Plant Abiotic Stress Research Group & Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City 70000, Vietnam;6. Signaling Pathway Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumiku, Yokohama 230-0045, Japan;1. National Field Genebank for Tropical Fruits, South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong Province 524091, PR China;2. School of Life Sciences, University of Shaoxing, Zhejiang Province 312000, PR China;3. School of Life and Environmental Sciences, Deakin University, Geelong Campus at Waurn Ponds, Victoria 3217, Australia;1. The Wyler Department of Dryland Agriculture, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Israel;2. The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel;3. Northern R&D, Migal, Israel;1. Department of Plant Biotechnology, University of Szczecin, W?ska 13, 71-415 Szczecin, Poland;2. Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Pozuelo de Alarcón, Madrid, Spain;1. United Graduate School of Agricultural Science, Gifu University, Gifu 501-1193, Japan;2. Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan |
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| Abstract: | Bayoud, caused by Fusarium oxysporum f. sp. albedinis (Foa), is the most damaging disease of date palm in Morocco. In the present study we have investigated the effect of jasmonic acid (JA) on two defence-related enzymes, namely peroxidases (POX) and polyphenoloxidases (PPO) in date palm seedlings root. Our data show that exogenous application of JA at a concentration of 50 μM increased the activity of both enzymes. The increase of POX activity in the presence of JA was much more important than that observed following infection with the pathogen. As compared to untreated plants, PPO activity was 2.2 and 1.3 times higher in BSTN and JHL cultivars respectively. In addition, PAGE analysis revealed increased band intensity of the major constitutive isoforms of POX and PPO in both JA-treated and Foa-treated seedlings. Close examination of symptomatic and asymptomatic plants showed that root tissues of symptomatic plants were massively colonized by Foa. Also, disease development in these plants appeared to involve a marked degradation of the host cell walls early during the process of pathogen invasion. In contrast, the presence of Foa in asymptomatic plants induced limited necrotic lesions (hypersensitive-reaction like lesions) that were probably involved in reducing the progression of the pathogen. Together, our findings indicate that JA is capable of enhancing date palm root resistance to infection by Foa via the activation of defence-related enzymes such as PPO and POX. |
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