| 猪脂联素基因启动子克隆及多态性研究 |
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| 引用本文： | 凌飞,汪亮亮,杜红丽,李仕新,陈瑶生. 猪脂联素基因启动子克隆及多态性研究[J]. 安徽农业科学, 2010, 38(1): 413-415,418. DOI: 10.3969/j.issn.0517-6611.2010.01.034 |
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| 作者姓名： | 凌飞 汪亮亮 杜红丽 李仕新 陈瑶生 |
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| 作者单位： | 华南理工大学生物与工程学院,广东广州,510006;华南农业大学动物科学学院,广东广州,510642;华南农业大学动物科学学院,广东广州,510642;华南理工大学生物与工程学院,广东广州,510006;中山大学生命科学学院,广东广州,510006 |
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| 基金项目： | 农业部“948”项目资助(2006-G50) |
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| 摘 要： | [目的]为脂联素基因作为脂肪沉积候选基因的研究提供依据。[方法]通过猪BAC文库筛选及primer—walking的测序方法获得脂联素基因的启动子序列，采用PCR—RFLP技术对蓝塘猪、大花白猪、大白猪、长白猪和杜洛克猪5个品种共290头猪的脂联素基因启动子区的多态性进行了分析。[结果l脂联素基因的5’侧翼区-1010bp（G／A）的SNP位点，本地猪种中GG基N型频率明显高于外来猪种；-394bp（T／C）的SNP位点，本地猪种基因型分布较为丰富，而在外来猪种申却没有检测到CC基因型，且外来猪中T等位基因频率较高。[结论]脂联素基因上游-1010bp（G／A）的SNP位点突变可能会引起该基因转录水平的改变，而-394bp（T／C）的SNP位点与基因转录水平及与脂肪沉积可能无关．
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| 关 键 词： | 猪BAC文库 脂联素基因 启动子 SNP |
Cloning of the Porcine adiponectin Promoter and Study on Genetic Polymorphism |
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| LING Fei et al. Cloning of the Porcine adiponectin Promoter and Study on Genetic Polymorphism[J]. Journal of Anhui Agricultural Sciences, 2010, 38(1): 413-415,418. DOI: 10.3969/j.issn.0517-6611.2010.01.034 |
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| Authors: | LING Fei et al |
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| Affiliation: | School of Biology and Engineering;South China University of Technology;Guangzhou;Guangdong 510006 |
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| Abstract: | [Objective] The aim was to provide basis for study on adiponectin as candidate gene of fat deposition.[Method] By screening porcine BAC library and using primer-walking method,the promoter sequence of adiponectin was obtained.The polymorphisms of 290 pigs were analyzed with PCR-RFLP technology,including Lantang pig,Large spotted pig,Large white pig,Landrace and Duroc.[Result] At SNP site of adiponectin 5'-flanking region-1 010 bp(G/A),GG genotype frequency in Chinese indigenous pigs was significantly higher... |
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| Keywords: | Porcine BAC library Adiponectin Promoter SNP |
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