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猪脂联素基因启动子克隆及多态性研究
引用本文:凌飞,汪亮亮,杜红丽,李仕新,陈瑶生. 猪脂联素基因启动子克隆及多态性研究[J]. 安徽农业科学, 2010, 38(1): 413-415,418. DOI: 10.3969/j.issn.0517-6611.2010.01.034
作者姓名:凌飞  汪亮亮  杜红丽  李仕新  陈瑶生
作者单位:华南理工大学生物与工程学院,广东广州,510006;华南农业大学动物科学学院,广东广州,510642;华南农业大学动物科学学院,广东广州,510642;华南理工大学生物与工程学院,广东广州,510006;中山大学生命科学学院,广东广州,510006
基金项目:农业部“948”项目资助(2006-G50)
摘    要:[目的]为脂联素基因作为脂肪沉积候选基因的研究提供依据。[方法]通过猪BAC文库筛选及primer—walking的测序方法获得脂联素基因的启动子序列,采用PCR—RFLP技术对蓝塘猪、大花白猪、大白猪、长白猪和杜洛克猪5个品种共290头猪的脂联素基因启动子区的多态性进行了分析。[结果l脂联素基因的5’侧翼区-1010bp(G/A)的SNP位点,本地猪种中GG基N型频率明显高于外来猪种;-394bp(T/C)的SNP位点,本地猪种基因型分布较为丰富,而在外来猪种申却没有检测到CC基因型,且外来猪中T等位基因频率较高。[结论]脂联素基因上游-1010bp(G/A)的SNP位点突变可能会引起该基因转录水平的改变,而-394bp(T/C)的SNP位点与基因转录水平及与脂肪沉积可能无关.

关 键 词:猪BAC文库  脂联素基因  启动子  SNP

Cloning of the Porcine adiponectin Promoter and Study on Genetic Polymorphism
LING Fei et al. Cloning of the Porcine adiponectin Promoter and Study on Genetic Polymorphism[J]. Journal of Anhui Agricultural Sciences, 2010, 38(1): 413-415,418. DOI: 10.3969/j.issn.0517-6611.2010.01.034
Authors:LING Fei et al
Affiliation:School of Biology and Engineering;South China University of Technology;Guangzhou;Guangdong 510006
Abstract:[Objective] The aim was to provide basis for study on adiponectin as candidate gene of fat deposition.[Method] By screening porcine BAC library and using primer-walking method,the promoter sequence of adiponectin was obtained.The polymorphisms of 290 pigs were analyzed with PCR-RFLP technology,including Lantang pig,Large spotted pig,Large white pig,Landrace and Duroc.[Result] At SNP site of adiponectin 5'-flanking region-1 010 bp(G/A),GG genotype frequency in Chinese indigenous pigs was significantly higher...
Keywords:Porcine BAC library  Adiponectin  Promoter  SNP  
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