| 蒙古羊BMP15全长DNA序列的克隆与分析 |
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| 引用本文： | 刘永斌,荣威恒,王峰,何小龙,田春英. 蒙古羊BMP15全长DNA序列的克隆与分析[J]. 华北农学报, 2009, 24(1) |
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| 作者姓名： | 刘永斌 荣威恒 王峰 何小龙 田春英 |
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| 作者单位： | 内蒙古农牧业科学院,内蒙古,呼和浩特,010030;内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古农牧业科学院,内蒙古,呼和浩特,010030 |
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| 摘 要： | BMP15基因主要在哺乳动物卵巢中表达,对卵泡的发育和分化起重要作用.根据其他物种BMP15基因的保守序列设计特异性引物,扩增蒙古羊的全长DNA序列.从蒙古羊的血液中提取总DNA,采用PCR技术扩增出蒙古羊BMP15 DNA序列.将此片段克隆到PMD18-T载体中,经菌落PCR鉴定和DNA序列测定分析验证,证实所克隆序列BMP15为骨形态发生蛋白,符合骨形态发生蛋白基因的特征.序列分析表明,该DNA包含两个外显子序列和一个内含子序列以及两端的非翻译序列,全长6 642 bp,编码393个氨基酸,蒙古羊与牛的同源性最高(98.6%),与猪的同源性为90.5%.
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| 关 键 词： | 蒙古羊 BMP15 PCR 克隆 序列分析 |
Analysis and Cloning of Mongolian Sheep Bone Morphogenetic Protein 15 Gene Full DNA |
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| LIU Yong-bin,RONG Wei-heng,WANG Feng,HE Xiao-long,TIAN Chun-ying. Analysis and Cloning of Mongolian Sheep Bone Morphogenetic Protein 15 Gene Full DNA[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(1) |
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| Authors: | LIU Yong-bin RONG Wei-heng WANG Feng HE Xiao-long TIAN Chun-ying |
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| Affiliation: | 1.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences;Huhhot 010030;China2.College of Animals Science and Medicine;Inner Mongolia Agriculture University;Huhhot 010018;China; |
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| Abstract: | BMP15 has a important role to the development and differentiation of follicular and is specialized expressed in mammalian ovary.Specific primers was designed based on conserver sequence of the other animals,firstly,total DNA was extracted from blood of Mongolian sheep and the BMP15 DNA was amplified by PCR,The purified-PCR.product was cloned into PMD18-T vector.The results demonstrated that the Mongolian sheep BMP15 is belong to the family of bone morphogenetic protein.On the basis of comparing the sequence... |
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| Keywords: | BMP15 PCR |
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