| GO基因对草莓遗传转化及抗病性鉴定 |
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| 引用本文： | 金万梅,尹淑萍,鲁韧强,袁维风,董静,王桂霞.GO基因对草莓遗传转化及抗病性鉴定[J].分子植物育种,2005,3(6):797-800. |
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| 作者姓名： | 金万梅 尹淑萍 鲁韧强 袁维风 董静 王桂霞 |
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| 作者单位： | 北京市农林科学院林业果树研究所,北京,100093;北京市农林科学院林业果树研究所,北京,100093;北京市农林科学院林业果树研究所,北京,100093;北京市农林科学院林业果树研究所,北京,100093;北京市农林科学院林业果树研究所,北京,100093;北京市农林科学院林业果树研究所,北京,100093 |
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| 摘 要： | 利用PCR技术从黑曲霉（Aspergillus niger）中扩增并克隆了葡萄糖氧化酶（glucose oxidase,简称GO）基因,并将马铃薯病原诱导型启动子（Prp1-1基因启动子）与其融合构建了植物表达载体pCAMGO,经农杆菌介导转化获得了转基因草莓.在病原物灰霉菌孢子诱导后,由淀粉-KI的显色反应证实,转基因草莓中GO基因的表达引起H2O2的生成;用草莓灰霉病病原侵染,结果表明：转基因草莓抗灰霉病的能力较对照明显提高.
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| 关 键 词： | GO基因 草莓 遗传转化 抗病 |
GO Gene Transformation of Strawberry and its Resistance to Gray Mold |
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| Jin Wanmei,Yin Shuping,Lu Renqiang,Yuan Weifeng,Dong Jing,Wang Guixia.GO Gene Transformation of Strawberry and its Resistance to Gray Mold[J].Molecular Plant Breeding,2005,3(6):797-800. |
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| Authors: | Jin Wanmei Yin Shuping Lu Renqiang Yuan Weifeng Dong Jing Wang Guixia |
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| Abstract: | Glucose oxidase (GO) gene was cloned from Aspergillus niger by PCR. Pl ant expressing vector was constructed with pathogeny inducing promer( Prp1-1 gen e) and GO gene. GO gene was introduced into strawberry mediated by Agrobaterium Tumefaciens. Transgenic strawberry released more H2O2 than that of control after induced by spores of graymold; disease resistance of transgenic strawberry was increased more than controlled plants. |
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| Keywords: | GO gene Strawberry Genetic Transformation Resistance to gray mold |
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