Preparation of additional monoclonal antibodies for detection and discrimination of potato virus Y isolates infecting potato |
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Authors: | A. Sanz M. Cambra C.Perez De San Roman J. G. Miguet E. Cortes M. T. Gorris C. Vela |
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Affiliation: | (1) Immunología y Genética Aplicada S.A. (INGENASA), Hermanos Garcia Noblejas 41, 28037 Madrid, Spain;(2) Instituto Valenciano de Investigaciones Agrarias (IVIA), Apdo. Oficial, 46113 Moncada Valencia, Spain;(3) Estación de Mejora de la Patata, Gobierno Vasco, Apdo. 46, 01080 Vitoria-Gasteiz, Spain |
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Abstract: | Summary Monoclonal antibodies (MAb) specific to potato virus Y (PVY) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate Bintje PVYo. Six MAb were characterized. The reactions of the MAb have been assayed by indirect double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-IDAS) against forty-nine PVY isolates and other potyviruses, representative of different potato areas in the world. All MAb reacted to at least some isolates in group O+C in ELISA-IDAS. One MAb did not react to any group N isolates. MAb 10E3 reacted to all isolates tested. Using standard double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-DAS), two MAbs did not react to any isolates, one reacted to some isolates in group O+C and MAb 10E3 reacted to all isolates but two in group C. A mixture of MAb 10E3 and 1E10 detected all isolates in ELISA-DAS and sensitivity was improved over that obtained with polyclonal antibodies from antisera. |
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Keywords: | ELISA serotyping pepper |
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