3株O3血清型鳗弧菌灭活疫苗的免疫原性和免疫保护效果 Immunogenicity and Protective Efficacy of Three Inactivated Vibrio anguillarum Serotype O3 Vaccine Preparations期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

3株O3血清型鳗弧菌灭活疫苗的免疫原性和免疫保护效果

引用本文：	李淑芳,唐磊,李杰,李贵阳,王晓冉,王小鹿,丁山,莫照兰. 3株O3血清型鳗弧菌灭活疫苗的免疫原性和免疫保护效果[J]. 渔业科学进展, 2018, 39(1): 137-143

作者姓名：	李淑芳唐磊李杰李贵阳王晓冉王小鹿丁山莫照兰

作者单位：	青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；上海海洋大学水产与生命学院上海 201306,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；中国海洋大学海洋生命学院青岛 266003,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；中国海洋大学海洋生命学院青岛 266003,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；中国海洋大学海洋生命学院青岛 266003,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；上海海洋大学水产与生命学院上海 201306,青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室农业部海水养殖病害防治重点实验室中国水产科学研究院黄海水产研究所青岛 266071；上海海洋大学水产与生命学院上海 201306

摘要：	鳗弧菌(Vibrio anguillarum)O3血清型菌株是感染鱼类的重要病原菌,本文研究了3株O3血清型鳗弧菌(SMP1、SMP3和SMP4)灭活疫苗的免疫原性和免疫保护。首先在牙鲆(Paralichthys olivaceus)体内对3株鳗弧菌进行复壮;检测复壮后的菌株毒力,检测的3株鳗弧菌对蓝蔓龙(Trichogaser trichopterus)的半数致死量(LD50)分别为105.1 CFU/ml(SMP1)、104.7 CFU/ml(SMP3)和105.4 CFU/ml(SMP4);制备了3株菌的甲醛灭活疫苗,注射免疫牙鲆,免疫后第7天可检测到牙鲆的血清特异性抗体产生,免疫后第28天的血清特异性抗体效价为1:1280(SMP1)、1:640(SMP3)和1:905(SMP4),提供的免疫保护率(Relative percent survival rate,RPS)为94.4%(SMP1)、100%(SMP3)和73.7%(SMP4)。研究表明,3株致病性O3血清型鳗弧菌菌株具有良好的免疫原性,其中SMP3为最适疫苗候选株。本研究为鳗弧菌O3血清型疫苗的开发应用奠定了基础。
关键词：	鳗弧菌O3血清型；LD50；灭活疫苗；血清抗体效价；免疫保护率
收稿时间：	2016-11-06
修稿时间：	2016-12-29
Immunogenicity and Protective Efficacy of Three Inactivated Vibrio anguillarum Serotype O3 Vaccine Preparations

LI Shufang,TANG Lei,LI Jie,LI Guiyang,WANG Xiaoran,WANG Xiaolu,DING Shan and MO Zhaolan. Immunogenicity and Protective Efficacy of Three Inactivated Vibrio anguillarum Serotype O3 Vaccine Preparations[J]. Progress in Fishery Sciences, 2018, 39(1): 137-143

Authors:	LI Shufang TANG Lei LI Jie LI Guiyang WANG Xiaoran WANG Xiaolu DING Shan MO Zhaolan

Affiliation:	Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;College of Marine Life Sciences, Ocean University of China, Qingdao 266003,Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;College of Marine Life Sciences, Ocean University of China, Qingdao 266003,Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;College of Marine Life Sciences, Ocean University of China, Qingdao 266003,Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306 and Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306

Abstract:

Keywords:	Vibrio anguillarum serotype O3 LD50 Inactivated vaccine Serum antibody titer Relative percent survival rate
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