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不同稀释液对Beagle犬精液保存效果的比较
引用本文:张志光,易磬源,刘清神,刘运忠.不同稀释液对Beagle犬精液保存效果的比较[J].广东畜牧兽医科技,2013(6):37-40.
作者姓名:张志光  易磬源  刘清神  刘运忠
作者单位:[1]华南农业大学动物科学学院,广东广州510642 [2]广州医药研究总院,广东广州510240
基金项目:广东省科技计划项目(2011B060300001;2011B020306008)
摘    要:通过比较4种不含卵黄的犬精液稀释保存液在低温环境中(4℃)的实际使用效果,筛选出适合的不含卵黄低温稀释保存配方.不含卵黄的配方Ⅳ (Tris 2.108 g,柠檬酸水合物1.2g,果糖1.0g,青霉素100 mg,双氢链霉素100 mg,牛血清白蛋白(BSA)4 g,加蒸馏水到100 mL)具有较好的低温稀释保存效果,并首次提出牛血清白蛋白(BSA)可作为非常时期犬精液低温稀释液中卵黄的替代物.

关 键 词:beagle犬  精液  稀释液  低温(4℃)

Effect of Different Extenders on Preservation of Semen from Beagle Dogs
Zhang Zhiguang Yi Qingyuan Liu Qingshen Liu Yunzhong.Effect of Different Extenders on Preservation of Semen from Beagle Dogs[J].Guangdong Journal of Animal and Veterinary Science,2013(6):37-40.
Authors:Zhang Zhiguang Yi Qingyuan Liu Qingshen Liu Yunzhong
Institution:Zhang Zhiguang Yi Qingyuan Liu Qingshen Liu Yunzhong( 1 .College of Animal Science, South China Agricultural University, Guangzhou 510642, China; 2.Guangzhou General Pharmaceutical Research Institute, Guangzhou 510240,China )
Abstract:After compared the actual use effect of four kinds of non egg yolk extenders in low temperature (4 ~C ), the suitable extenders in low temperature (4℃) which include non egg yolk was chosen.The results showed that,the non egg yolk extender IV (Tris 2.108g, Citric acid1.2g, fructose lg, penicillin(100mg), dihydrostreptomycin(100mg), bovine serum albumin(BSA) 4g,add distilled water to 100mL)had better preservation effect in low temperature,and first proposed that bovine serum albumin(BSA) could replace the egg yolk in preserving beagle canine semen in emergency time.
Keywords:beagle  canine semen  extender  low temperature
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