应用多重荧光PCR快速筛查作物中转基因成分研究

通过对我国批准进口的和获得农业转基因生物安全证书的转基因玉米转化体的序列分析发现,除DAS40278-9和BLVA430101外,CaMV35s启动子、NOS终止子、CrylAb/Ac基因和pat基因覆盖了 21种玉米转基因转化体.通过引物组合筛选、反应体系优化、灵敏度测试、适用性测试等实验,建立了基于4个通用筛选元件...

Rapid Screening of Genetically Modified Ingredient in Crops by Multiplex Real-time PCR

Through sequence analysis of genetically modified corn transformants approved for import and obtained agricultural genetically modified organisms safety certificates in China, it was found that CaMV35s promoter, NOS terminator, Cry1Ab/Ac gene, and Pat gene covered 21 corn transgenic events. In this study, a series of experiments, including primer combination screening, reaction system optimization, sensitivity test, and applicability test were conducted. Consequently, a five-plex fluorescent PCR system and a duplex fluorescent PCR system were established based on four common screening elements and an endogenous gene zSSIIb. The measurement results of parameters showed that these two multiplex fluorescent PCR detection systems established in this study are characterized by strong specificity, good stability high sensitivity, which can reach 0.05%. Meanwhile, these two systems can also be used to screen and identify genetically modified ingredients in soybean, rice and many other crops.