高效液相色谱-氢化物-原子荧光联用技术测定海藻中无机砷

本文采用高效液相色谱-氢化物发生-原子荧光联技术（ HPLC-HG-AFS）对海藻中的无机砷进行检测。以藻类中羊栖菜的无机砷含量为指标,确定提取剂的种类和浓度,并通过曲面拟合的方法确定了最佳提取温度和提取时间。结果表明,以1．0%HCL为提取剂,提取温度52℃,提取时间78 min的效果最佳。该方法无机砷的检出限为0．0628 mg/kg,样品加标量为0．2 mg/kg 和1．0 mg/kg 时回收率在80．5%~105．7%之间,相对标准偏差（RSD, n=6）在4．2%~5．3%之间。并应用此分析技术测定了福建省内60个不同产地紫菜样品中无机砷含量,结果表明,主要生产区紫菜中无机砷含量很低,仅占总砷含量的0~1．96%。

Determination of inorganic arsenic in seaweeds with HPLC-HG-AFS

This paper is about the research of the determination of inorganic arsenic in edible seaweeds with High performance liquid chromatography-hydride generation-atomic fluorescence spectrometry （ HPLC-HG-AFS） . The curved surface fitting method was applied to establish optimum conditions of extraction reagent, time and temperature based on the inorganic arsenic contents of Sargassum fusiforme. It was found that the op-timum conditions is with 0. 1%HCL at 52℃ for 78 min. The detection limit of methodology was 0. 0 628 mg/kg. The recovery rates of 1. 0 mg/kg and 10. 0 mg/kg in samples were between 80. 5% and 105. 7%, and the relative standard deviations were between 4. 5% and 5. 0%. The content of inorganic arsenic of sixty types of laver from different areas in Fujian Province was examined. The results showed that inorganic arsenic con-centration in laver was below, which accounted for the total arsenic concentration of 0~1. 96%.