| 彩色马蹄莲“柠檬”组织培养研究 |
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| 引用本文： | 束晓春,彭峰,何树兰,李乃伟,夏冰. 彩色马蹄莲“柠檬”组织培养研究[J]. 江苏林业科技, 2009, 36(2): 28-30 |
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| 作者姓名： | 束晓春 彭峰 何树兰 李乃伟 夏冰 |
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| 作者单位： | 江苏省,中国科学院植物研究所,江苏,南京,210014 |
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| 摘 要： | 以彩色马蹄莲品种“柠檬”的块茎为外植体,研究不同的消毒方法和激素水平对彩色马蹄莲离体繁殖的影响,并筛选适合彩色马蹄莲试管苗生长的培养基。结果表明,在初代试验中对彩色马蹄莲的块茎消毒以0.2%升汞处理15 min为宜;MS＋1.0-2.0 mg/L6-BA＋0.1-0.2 mg/L NAA为适宜的愈伤组织诱导培养基;在继代培养基MS＋0.5 mg/L6-BA＋0.1 mg/L NAA上,愈伤组织能够分化出状态良好的丛生芽并继续增殖;在培养基MS＋0.1mg/L IBA上,生根率达100%,试管苗生长健壮,根系良好。
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| 关 键 词： | 彩色马蹄莲 组织培养 繁殖 |
The tissue culture and rapid propagation of Zantedeschia hybrida 'Ning Meng' |
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| SHU Xiao-chun,PENG Feng,HE Shu-lan,LI Nai-wei,XIA Bing. The tissue culture and rapid propagation of Zantedeschia hybrida 'Ning Meng'[J]. Journal of Jiangsu Forestry Science & Technology, 2009, 36(2): 28-30 |
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| Authors: | SHU Xiao-chun PENG Feng HE Shu-lan LI Nai-wei XIA Bing |
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| Affiliation: | Institute of Botany;Jiangsu Province and the Chinese Academy of Sciences;Nanjing 210014;China |
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| Abstract: | In this tissue culture experiment, bulbs of Zantedeschia hybrida ＇ Ning Meng＇ were used as explants. The results showed that the best time of 0. 2% HgCl2 disinfectant was 15 min. and the best calli induction medium was MS ＋ 1.0 -2.0 mg/L 6-BA ＋ 0. 1 -0. 2 mg/L NAA. The calli would grow into cluster buds in the medium of MS ＋ 0. 5 mg/L 6-BA ＋0. 1 mg/L NAA, and the buds grew well with new callus proliferation. The rooting rate was 100% in the medium of MS ＋0. 1 mg/L IBA. |
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| Keywords: | Zantedeschia hybrida Tissue culture Rapid propagation |
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