| 禾谷缢管蚜羧酸酯酶基因cDNA片段的克隆与序列分析 |
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| 引用本文： | 陈茂华,蓝家样,韩召军,乔宪凤,曲明静. 禾谷缢管蚜羧酸酯酶基因cDNA片段的克隆与序列分析[J]. 麦类作物学报, 2006, 26(4): 145-148 |
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| 作者姓名： | 陈茂华 蓝家样 韩召军 乔宪凤 曲明静 |
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| 作者单位： | 南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095;湖北省农科院经济作物研究所,湖北,武汉,430064;湖北省农科院经济作物研究所,湖北,武汉,430064;南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095;南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095;山东花生研究所,山东,青岛,266100 |
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| 摘 要： | 为开展禾谷缢管蚜（Rhopalosiphum padi Linnaeus）抗性的分子生物学研究，设计简并引物并采用RT—PCR技术，克隆了长度分别为260、331、337bp的3个禾谷缢管蚜的羧酸酯酶cDNA片段，命名为Rp．est1、Rp．est2、Rp．est3；3个片段推导氨基酸残基分别为87、110、112个；同源性分析表明，Rp．estl、Rp．est2、Rp．est3之间的相似性在40％左右，表明其分别代表不同基因；Rp．est1、Rp．est2、Rp．est3与其他昆虫羧酸酯酶基因序列具有较高的相似性，其GeneBank登录号分别为AY622997、AY869713、AY869714。
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| 关 键 词： | 禾谷缢管蚜 羧酸酯酶 基因克隆 序列分析 |
| 文章编号： | 1004-1389（2006）04-0145-04 |
| 收稿时间： | 2005-12-05 |
| 修稿时间： | 2006-03-16 |
Cloning and Sequence Analysis of Carboxylesterase Gene cDNA Fragments from Rhopalosiphum padi Linnaeus |
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| CHEN Mao-hu,LAN Jia-yang,HAN Zhao-jun,QIAO Xian-feng,QU Ming-jing. Cloning and Sequence Analysis of Carboxylesterase Gene cDNA Fragments from Rhopalosiphum padi Linnaeus[J]. Journal of Triticeae Crops, 2006, 26(4): 145-148 |
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| Authors: | CHEN Mao-hu LAN Jia-yang HAN Zhao-jun QIAO Xian-feng QU Ming-jing |
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| Affiliation: | 1. Key lab. of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture, Naniing Agricultural University, Nanjing , Jiangsu 210095, China;2. Instiute of Economic Crops, Hubei Academy of Agricultural science, Wuhan, Hubei 430064,Chlna; 3. Shandong Institute of Peanut, Qingdao, Shandong 266100, China |
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| Abstract: | Using RT-PCR technique with degenerate primers,3 carboxylesterase gene cDNA fragment(Rp.est1,Rp.est2,Rp.est3) were cloned from Rhopalosiphum padi(Linnaeus),which were 260 bp,331 bp,337 bp nucleotides in length,and encodes 87,110,112 amino acids,respectively.Sequence analysis showed that the 3 cloned carboxylesterase gene cDNA fragments show near 40% homology each other,and have high homology with those from other insects.The GeneBank accession numbers of the 3 carboxylesterase genes are AY622997,AY869713,AY869714. |
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| Keywords: | Rhopalosiphum padi Linnaeus Carboxylesterase Geneclone Sequence analysis |
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