突变型抑癌基因p53在鸡马立克氏病肿瘤组织中的表达研究

[目的]探讨抑癌基因p53在鸡马立克氏病的发生与发展中的作用和意义。[方法]采用免疫组织化学和细胞化学技术,观察抑癌基因p53在鸡马立克氏病肿瘤组织及体外培养感染马立克病毒的鸡胚成纤维细胞中的表达。[结果]在体外培养的鸡胚成纤维细胞(CEF)感染马立克氏病毒后,感染的CEF呈阳性。在马立克氏病的发生过程中,突变型抑癌基因p53在病鸡的肝脏、肾脏、肿瘤、心脏、脾脏、肺脏、胸腺及法氏囊中均可检出中等量的表达。p53发生突变时所产生的p53蛋白突变体的半衰期比未发生突变时要长。[结论]突变型p53基因具有直接转化细胞和阻碍野生型p53发挥作用的特点,致使突变的DNA得以积累,最终导致癌变的发生。

Study on the Expression of Mutant Tumor Suppressor Gene p53 in the Tumor Tissues in Chicken with Marek's Disease

[Objective] The research aimed to discuss the role and significance of tumor suppressor gene p53 in the occurrence and development of Marek's disease in chicken.[Method] The expression of tumor suppressor gene p53 in the tumor tissues in chicken with Marek's disease and chicken embryo fibroblasts in vitro infected by Marek' s disease virus were observed by using immunohistochemical and cytochemical techniques.[Result] After chicken embryo fibroblasts(CEF) cultured in vitro was infected by Marek' s disease virus,the infected CEF showed to be positive.In the occurrence process of Marek's disease,the moderate expression of mutant suppressor gene p53 could be detected in the liver,kidney,tumor,heart,spleen,lung,thymus and bursa of bursa of fabricius in diseased chicken.The half life of the produced p53 protein mutant when the mutation of p53 was occurred was longer than that without the occurrence of mutations.[Conclusion] Mutant p53 had the characteristics of transforming cells directly and hindering the action played by wild-type p53 gene,which caused the accumulation of DNA mutant and finally led to the carcinogenesis.