引进国外马铃薯种质资源的遗传多样性分析

应用RAPD标记技术研究国外马铃薯品种的遗传多样性,以期明确这些品种的亲缘关系,为马铃薯优良品种的选育提供理论依据。利用筛选的29条RAPD引物,对24份国外马铃薯品种进行遗传多样性分析。结果显示,29条RAPD引物共扩增出165个条带,其中116条为多态性条带,多态性比例为70.3%;试供的24个国外马铃薯品种间的遗传相似系数在0.514~0.816之间,平均为0.677;相似系数小于0.6的品种对仅为7.25%;24个马铃薯品种可聚为2大类6亚类。结果表明,试供的24个国外马铃薯品种间的遗传相似性高,遗传基础比较狭窄。

Genetic Diversity Analysis of Potato Germplasm Resources from Abroad

The random amplified polymorphic DNA procedure (RAPD) was used to study the genetic diversity of potato varieties abroad, which were to clear the genetic relationship of these varieties and provide the theoretical basis for selecting and breeding of fine potato varieties. The genetic diversity of 24 potato varieties abroad were analyzed using 29 selected RAPD primers. The result showed that there were 165 bands amplified with 29 RAPD primers, in which 116 bands were polymorphic, and the polymorphic rate was 70.3%; The coefficients of genetic similarity among 24 potato varieties were ranged from 0.514 to 0.816, with an average of 0.667; The variety pairs with similarity coefficients less than 0.6 only accounted for 7.25%; 24 potato varieties could be divided into 2 classes and 6 subclasses. These results suggest that the genetic similarity is high and the genetic base is narrow among 24 potato varieties abroad.