外源物质对半夏愈伤氮代谢关键酶及有效成分积累的影响

以生长均一且旺盛的半夏愈伤组织为材料,研究外源添加物苯丙氨酸(Phe),天冬氨酸(Asp),硝普钠(SNP),乙酰水杨酸(ASA)单独或两两复合对半夏愈伤组织氮代谢关键酶及有效成分含量的影响.结果表明:单独添加外源物均可有效提高氮代谢关键酶及PAL的活性、可溶性蛋白及总生物碱的含量,SNP尤其明显;复合添加外源物时,1mg/L ASA和150mg/L Asp复合加入时诱导氮代谢关键酶及PAL的活性升高且具有显著性,可溶性蛋白、总生物碱、鸟苷含量显著增加.50mg/L Phe+1mg/L ASA复合加入时腺苷含量比对照提高了128.6%.说明外源添加物能有效调控次生代谢过程中氮代谢相关酶的变化,从而影响半夏愈伤组织中次生代谢产物含量.

Effects of Exogenous Substances on the Key Enzymes Involved in Nitrogen Metabolism in Pinellia ternate Calli and on the Accumulation of Active Components in Them

In this study, we took uniform and vigorous calli of Pinellia ternate as the experiment materials and studied the effects of exogenous substances (Phe, Asp, SNP and ASA) used alone or in combination on the key enzymes involved in N metabolism and the accumulations of active components in them. The results showed that the activities of nitrogen metabolism-involved key enzymes and the contents of soluble protein and total alkaloids in the calli were increased in all treatments, where an exogenous substance was applied, the effect of SNP being most significant. In treatments, where two exogenous substances were added into the medium, the activities of N metabolism-involved key enzymes and PAL as well as the contents of soluble protein, total alkaloids and guanosine were increased significantly after the addition of 1 mg/L ASA+150 mg/ L Asp. In the treatment of 50 mg/L Phe+1 mg/L ASA, the contents of adenosine increased by 129%, compared with the control. To sum up, exogenous substances can effectively regulate the activities of nitrogen metabolism-related enzymes of secondary metabolism, thus affecting the contents of secondary metabolites of the calli of P. ternate.