| Cloning and Sequence Analysis of Glycoprotein D Gene of Bovine Herpesvirus-1 Strain Luojing |
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| 作者姓名： | LIJi-chang TONGGuang-zhi QIUHua-Ji ZHOUYan-Jun XUEQiang |
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| 作者单位： | [1]NortheastAgriculturalUniversity,Harbin,Heilongjiang,150030,PRC [2]HarbinVeterinaryResearchInstitute.Harbin,Heilongjiang,150001,PRC |
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| 摘 要： | By means of PCR,the gene encoding gD of bovine herpesvirus-1 (BHV-1) strain Luojing was amplified,cloned and sequenced. The nucleotide sequence of this gD gene was 1 251 bp,encoding 417 amino acids. Comparied with the published P8-2 strain ,the homology of the necleotide sequence is 99.92%,and that of the deduced amino acid sequence is 100%. The results indicated that gD of BHV-1 was highly conservative.
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| 关 键 词： | 糖蛋白 D 基因 克隆 序列分析 牛 疱疹病毒 菌株 PCR 氨基酸 糖苷 |
Cloning and Sequence Analysis of Glycoprotein D Gene of Bovine Herpesvirus-1 Strain Luojing |
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| LIJi-chang TONGGuang-zhi QIUHua-Ji ZHOUYan-Jun XUEQiang.Cloning and Sequence Analysis of Glycoprotein D Gene of Bovine Herpesvirus-1 Strain Luojing[J].Journal of Northeast Agricultural University,2003,10(2):137-140. |
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| Authors: | LI Ji-chang TONG Guang-zhi QIU Hua-ji ZHOU Yan-jun XUE Qiang |
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| Abstract: | By means of PCR,the gene encoding gD of bovine herpesvirus-1 (BHV-1) strain Luojing was amplified,cloned and sequenced.The nucleotide sequence of this gD gene was (1 251 bp,)encoding 417 amino acids.Comparied with the published P8-2 strain,the homology of the necleotide sequence is 99.92%,and that of the deduced amino acid sequence is 100%.The results indicated that gD of BHV-1 was highly conservative. |
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| Keywords: | bovine herpesvirus-1(BHV-1) D glycoprotein gene(gD) cloning sequence analysis |
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