Coleoptile cuticle of barley is necessary for the increase in appressorial turgor pressure of Blumeria graminis for penetration |
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Authors: | Mana Iwamoto Yoji Takeuchi Yasuhiro Takada Shintaro Kohno Isao Matsumoto Naoto Yamaoka |
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Affiliation: | (1) Cell Function and Structure, Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan;(2) Laboratory of Plant Pathology, Department of Agriculture, Ehime University, Matsuyama 790–8566, Japan |
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Abstract: | To determine whether the cuticle of the barley coleoptile is responsible for a rise in appressorial turgor pressure in Blumeria graminis, we determined the appressorial turgor pressure by measuring cytorrhysis and plasmolysis in the presence of PEG6000. Appressorial turgor pressure significantly increased 13–14 h after inoculation. On the other hand, when the cuticle was completely removed from the barley coleoptile surface with diethyl ether, turgor pressure did not increase. Moreover, when we then recoated the surface with the exogenous barley cuticle fraction, appressorial turgor pressure significantly increased 12–13 h after inoculation. These results suggest that the cuticle on the surface of the barley coleoptile is necessary for the increase in the appressorial turgor pressure. |
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Keywords: | Blumeria graminis Turgor pressure Appressorial germ tube |
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