Sources of resistance to Watermelon Chlorotic Stunt Virus in melon |
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Authors: | M. T. Yousif A. Kheyr-Pour B. Gronenborn M. Pitrat C. Dogimont |
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Affiliation: | National Institute for Promotion of Horticultural Exports, University of Gezira, Wad Medani, Sudan;;Institut des Sciences Végétales, CNRS, 91 198 Gif-sur-Yvette;;INRA, UR1052, Unitéde Génétique et d'Amélioration des Fruits et Légumes, Avignon, BP 94, F-84143 Montfavet, France;;Corresponding author, E-mail: |
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Abstract: | Watermelon Chlorotic Stunt Virus (WmCSV) is a bipartite geminivirus residing in the genus Begomovirus (family Geminiviridae) and is whitefly transmitted ( Bemisia tabaci ). WmCSV infects members of the Cucurbitaceae , and represents a considerable threat to melon and watermelon crops in Sudan, Yemen, and Iran. One hundred twenty-eight melon accessions, mostly collected from Sudan, were screened in the open-field in Sudan under severe whitefly inoculation pressure in order to identify sources of resistance to WmCSV. One WmCSV resistant line HSD 2445-005 selected from this first screening along with 22 new melon accessions were agro-inoculated with an infectious Sudanese clone of WmCSV. Ten accessions exhibited resistance to WmCSV infection upon agro-inoculation. Six of them were confirmed to have complete resistance to WmCSV through graft-inoculation as well as multiple field trials in Sudan. These lines include the Sudanese accession HSD 2445-005, the South African accession PI 282448, and three Indian accessions: 90625, PI 124112, and PI 414723. Resistant plants exhibited no disease symptoms, and virus DNA could not be detected by hybridization with a WmCSV-specific probe. |
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Keywords: | Cucumis melo Bemisia tabaci Begomovirus germplasm evaluation agro-inoculation |
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