六味地黄汤对发育迟缓胎鼠脑NGF、EGF表达影响

目的 观察补肾益髓经典方六味地黄汤对发育迟缓胎鼠脑神经生长因子（NGF）、表皮细胞生长因子（EGF）表达影响，并为宫细胞内脑发育迟缓的治疗提供支持。方法 10只孕鼠依照受孕先后顺序随机分成5组，正常组、模型组、六味地黄汤高剂量组、六味地黄汤中剂量组、六味地黄汤低剂量组，采用孕鼠被动吸烟复制胎鼠发育迟缓模型，正常组、模型组均灌胃蒸馏水，六味地黄汤高、中、低剂量组分别灌胃2.164、1.082、0.541 g/mL的六味地黄汤浓缩液。于孕19 d将5组孕鼠处死，称算每组活胎脑体质量比；采用免疫组化法检测胎鼠脑内NGF、EGF的表达。结果 被动吸烟可使胎鼠脑体质量比降低，六味地黄汤高剂量能增加脑体质量比；模型组NGF、EGF表达低于正常组（P<0.01），治疗组NGF、EGF表达均高于模型组（P<0.01或P<0.05）。结论 孕期被动吸烟可以导致胎鼠脑发育迟缓，六味地黄汤能通过调控脑内NGF、EGF改善胎鼠脑的发育。

Influence of Liuwei Dihuang Decoction on Exppression of NGF and EGF in Brain Tissue of Intrauterine Growth Retardation Rats

Obejective To observe Liuwei Dihuang Decoction on the expression of nerve growth factor (NGF) and epidermal growth factor (EGF) in brain tissue of intrauterine growth retardation rats and provide support for the treatment. Methods 10 pregnancy animals according to the order of ngravidation were randomly divided into 5 groups:nomal group, model group, high dose of Liuwei Dihuang Decoction (HLWDHD) group, medium dose of Liuwei Dihuang Decoction (MLWDHD) group, low dose of Liuwei Dihuang Decoction (LLWDHD) group. The IUGR model was established by passive smoking. Nomal group and model group were fed by water. The HLWDHD, MLWDHD and LLWDHD groups were given 2.164, 1.082, 0.541 g/mL concentrated solution of Liuwei Dihuang Decoction by gavage, respectively. On the 19th day of pregnancy, 10 pregnant rats were killed. The body and brain weight of lively fetal rats were scaled, then the expression of NGF and EGF were determined by immunohistochemical method. Results Passive smoking can decrease brain weight of fetal rats, while the HLWDHD group increase brain weight. The expression of NGF and EGF in model group was lower than nomal group (P<0.01), the expression of NGF and EGF in treatment group was higher than that in model group (P < 0.01 or P < 0.05). Conclusion The passive smoking during pregnance can lead to growth retardation, and Liuwei Dihuang Decoction can improve the growth of fetal rats by regulating NGF and EGF in brain.