咳喘宁对RSV诱发哮喘大鼠ICAM-1表达的影响

目的 研究咳喘宁对RSV诱发哮喘大鼠气道重塑形态学及ICAM-1表达的影响，阐明其防治哮喘的作用机制。方法 将60只幼年SD雄性大鼠随机分为6组，即正常组，模型组，泼尼松与沙丁胺醇治疗组（简称泼+沙治疗组），咳喘宁大、中、小剂量组，每组10只。用卵清蛋白复制大鼠哮喘模型，并予呼吸道合胞病毒（RSV）激发哮喘。大鼠处死后肺组织行HE染色观察炎症反应；图像分析软件测定支气管壁厚度及气道壁面积；免疫组化pv-9000二步法检测ICAM-1表达水平。结果 与模型组相比，各治疗组炎症反应较轻。各治疗组支气管壁厚度及气道壁面积改变较小，与模型组相比差异有统计学意义（P<0.01）；咳喘宁中剂量组较泼+沙治疗组作用略差，但差异无统计学意义（P>0.05）；咳喘宁大、小剂量组与泼+沙治疗组相比，其作用明显较差，有统计学意义（P<0.05）。各治疗组与模型组比较，其肺组织中ICAM-1表达均降低，有显著性差异（P<0.01）；泼+沙治疗组ICAM-1表达稍低于咳喘宁中剂量组，但差异无统计学意义（P>0.05﹚。结论 咳喘宁可有效抑制气道重塑，其机制可能与降低肺组织中ICAM-1表达有关。

Effect of KeChuanNing on Expression of ICAM-1 in Asthma Rats Induced by RSV

Objective To explore the effect of KeChuanNing on airway remodeling morphology and the expression of ICAM-1 in asthma rats induced by RSV,and clarify the mechanism of prevention and treatment.Methods The 60 SD male rats were randomly divided into 6 groups:normal group,model group,prednisone and salbutamoll treatment group (PS treatment group) and KeChuanNing high,medium and low dose groups,with 10 rats in each group.The asthma rats models were copied by ovalbumin and the asthma was stimulated by respiratory syncytial virus.The lung tissue pathological changes were observed by HE staining.The bronchial wall thickness and airway wall area were setermined by image analysis software.The expression of ICAM-1 was detected by using immunohistochemistry pv-9000 method.Results Compared with the model group,the inflammatory reaction in treatment groups was less;the bronchial wall thickness and airway wall area were smaller,the difference was statistically significant (P<0.01).The function of KeChuanNing medium dose group was slightly weak than the PS treatment group,but the difference was not statistically significant (P>0.05).Compared with PS treatment group,the effect of the KeChuanNing high,low dose group was poor and had statistical significance (P<0.05).Compared with the model group,the lung tissue ICAM-1 expression in the treatment groups was decreased,the difference was significant (P<0.01).The expression of ICAM-1 in PS treatment group was slightly lower than KeChuanNing medium dose group,but the difference was not statistically significant (P>0.05).Conclusion KeChuanNing could effectively inhibit airway remodeling,the mechanism may be related with inhibiting the expression of ICAM-1 in lung tissue.