芦笋种质资源的SRAP遗传多样性分析

为了更深入地揭示芦笋品种（系）的遗传多样性，本研究采用SRAP技术对国内外12 个芦笋栽培种和杂交种的遗传多态性进行分析。结果表明，从170 对引物中筛选出29 对多态性强、条带清晰的引物，每对引物产生12~36 条谱带，共产生551 条谱带，其中多态性条带343 条，占总带数的61.21%。平均每个引物扩增的DNA带数是20.41 条，多态性带数是12.7 条。采用NTSYSpc2.1 软件对SRAP扩增结果进行聚类分析，12 份芦笋材料的相似系数在0.39~0.97 之间，平均相似系数为0.69。在Coefficient=0.69处划等值线，可将12份芦笋材料划分为4类。第一类：‘鲁芦笋一号’、‘88-5’、‘格兰德’、‘07-1’和‘06-6’。第二类：‘阿波罗’、‘泽西巨人’和‘阿特拉斯’。第三类：‘西班牙达玛斯’。第四类：‘荷兰全雄’、‘UC800’和‘西德全雄’。

Genetic Diversity Analysis of Asparagus Based on SRAP Markers

This paper analyzed the genetic polymorphism of 12 asparagus cultivars and hybrids from home and abroad using SRAP method, so as to deeply reveal the genetic diversity of asparagus varieties. The results showed that 29 primers with high polymorphism and clear bands were screened from 170 primers, each of them amplified 12 to 36 bands and a total of 551 DNA fragments were obtained, 343 bands showed polymorphism in all fragments, accounted for 61.21%. The average number of DNA bands produced by each primer was 20.41 and polymorphic bands were 12.7. The result of genetic similarity analysis of 12 asparagus genotypes showed that the Nei’s coefficient ranged from 0.39 to 0.97, and the average Nei’s coefficient was 0.69. A DNA molecular dendrogram was established for 12 genotypes which were divided into 4 groups according to the coefficient of 0.69. The first group included‘Lu Asparagus No.1’,‘88-5’,‘Gland’,‘07-1’and‘06-6’. The second group included‘Apollo’,‘Jersey Giant’and‘Atlas’. The third group included‘Spanish Damas’. The forth group included‘Holland all male’and‘West Germany all male’.