银屑平丸对BALB/c小鼠银屑病样模型血清中IL-17、IL-23表达的影响

目的 建造BALB/c小鼠背部银屑病样模型，探讨中成药银屑平丸治疗银屑病的可能作用机制。方法 选取48只BALB/c小鼠，随机分为空白组、模型对照组、雷公藤组、银屑平丸高、中、低剂量组，8只/组。给予除空白组外其余小鼠背部皮肤外涂咪喹莫特软膏连续8 d，1次/d，建立模型小鼠。末次给药后，观察小鼠背部皮肤进行皮损面积和严重程度指数（psoriasis area and serenty index，PASI）评分；检测血清中IL-17、IL-23水平；取背部皮肤组织进行组织病理比较。结果（1）银屑平丸各组和雷公藤组对银屑病样小鼠背部皮肤PASI评分比较无明显的差异（P>0.05），而较模型对照组和空白组评分均有明显的差异（P<0.05）。（2）各组银屑病样小鼠外周血IL-17和IL-23含量明显高于空白组（P>0.05）。而银屑平丸各组和雷公藤组均与模型对照组外周血中含量有明显的差异（P<0.05）。（3）银屑平丸各组和雷公藤组对银屑病样小鼠背部皮肤组织病理变化较模型对照组和空白组有明显差异（P<0.05）。结论 银屑平丸改善银屑病模型小鼠的作用机制可能通过抑制IL-17、IL-23，起到减轻小鼠银屑病样皮损的发生、发展的作用。

Effects of Yinxieping Pills on IL-17, IL-23 Expression of in Serum of Psoriasis-like BALB/c Mice Models

Objective The construction of the back psoriasis-like BALB/c mice models discussing Yinxieping pills in the treatment of psoriasis may mechanism. Methods A total of 48 male BALB/c mice were randomly divided into blank control group, model control group, tripterygium wilfordii group, Yinxieping pills of high, medium and low dose groups, eight mice in each group. Except for blank group, the mice in other groups were given back skin of mice with imiquimod cream for 8 days, 1 times/d, the rat models were established. After the last administration, the psoriasis area and severity index (PASI) score of the skin in the back of mice was observed; the levels of IL-23 and IL-17 in serum were detected; the tissue pathology in back skin tissue was compared. Results (1) The PASI score of the skin in the back of psoriasis-like BALB/c mice of Yinxieping pill groups and tripterygium wilfordii group has no significant difference (P>0.05), and compared with the model control group and blank group, scores had significant differences (P<0.05). (2) The contents of IL-17 and IL-23 in the peripheral blood of mice were significantly higher than those in the blank group (P>0.05). Compared with model control group, the contents in peripheral blood of the patients in Yinxieping pill groups and triptolide group had significant diffrences (P<0.05). (3) The pathological changes of Yinxieping pill group and tripterygium wilfordii group on psoriasis-like mice back skin tissue had obvious changes compared with the model control group and blank control group (P<0.05). Conclusion The mechanism of Yinxieping pills improving psoriasis model mice may be by inhibiting IL-17, IL-23, to alleviate the occurrence and development.of psoriasis-like lesions in mice.