| 宠物龟眼炎的病原鉴定及临床药物治疗的研究 |
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| 引用本文： | 陈晨,潘连德.宠物龟眼炎的病原鉴定及临床药物治疗的研究[J].上海水产大学学报,2011(2). |
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| 作者姓名： | 陈晨 潘连德 |
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| 作者单位： | 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室; |
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| 基金项目： | 国家自然科学基金(39970582); 上海市教育委员会重点项目(07ZZ135); 上海市重点学科建设项目(Y1101) |
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| 摘 要： | 眼炎是宠物龟疾病中一种常见的疾病,具有闭眼、眼睑红肿、瞬膜异位等症状。从患有眼炎的宠物龟眼部分离出菌株A5,经人工回归感染试验确定其为眼炎的病原菌。综合该菌在形态、生理生化特征及16S rDNA测序分析等检验结果,确定A5菌株为荧光假单胞菌(Pseudomonas fluorescens bv.C)。药敏试验证实,该菌对氧氟沙星、庆大霉素、妥布霉素、氧哌嗪青霉素等抗生素敏感。对40个眼炎病例进行分组药物治疗,比较治疗方案,结果显示:氧氟沙星药效优于氯霉素与磺胺醋酰钠,养殖水温25℃治疗效果优于5℃,眼科手术剔除分泌物可缩短治疗时间。增加给药次数至6次/d、增加肌肉注射,疗效无显著性差异。此研究结果将为宠物龟眼科疾病的诊断与治疗起到指导性作用。
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| 关 键 词： | 宠物龟 眼炎 荧光假单胞菌 治疗 |
Identification of pathogen from pet chelonian with ophthalmia and the clinic therapy |
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| CHEN Chen,PAN Lian-de.Identification of pathogen from pet chelonian with ophthalmia and the clinic therapy[J].Journal of Shanghai Fisheries University,2011(2). |
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| Authors: | CHEN Chen PAN Lian-de |
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| Institution: | CHEN Chen,PAN Lian-de(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of Education,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | Symptoms of ophthalmia of pet chelonian included eye closed,eyelid got red and membranae nictitans moved.It is a common disease on pet chelonian.A dominant bacterial strain isolated from the sick chelonian's eye is assigned as the strain A5.The artificial infection test proved that the isolated A5 was the pathogenic bacterium that caused the disease.Synthesizing the morphology,physiological and biochemical characterization,and 16S rDNA of A5 was sequenced,the results showed that strain A5 exhibited the high... |
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| Keywords: | pet chelonian ophthalmia Pseudomonas fluorescens therapy |
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