| 榉树叶器官再生植株技术 |
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| 引用本文： | 刘海龙,张日清,汪灵丹,杨开太,黄金使,曾永明. 榉树叶器官再生植株技术[J]. 广西林业科技, 2013, 0(3): 231-233 |
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| 作者姓名： | 刘海龙 张日清 汪灵丹 杨开太 黄金使 曾永明 |
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| 作者单位： | [1]广西林业科学研究院国家林业局中南速生材繁育实验室广西优良用材林资源培育重点实验室南宁530002 [2]中南林业科技大学林学院,湖南长沙410004 |
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| 基金项目： | 国家林业公益性行业科研专项（200904011）;广西优良用材林资源培育重点实验室自主课题（1280301）;广西青年基金项目（2013GXNsFBA019093） |
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| 摘 要： | 以榉树（Zelkova schneideriana）叶片为外植体，先诱导愈伤组织的产生，再诱导愈伤组织分化出芽，研究榉树叶器官再生植株技术，结果表明：榉树叶片诱导出愈伤组织很容易，幼嫩叶片的诱导率可达到90％以上，而由愈伤组织诱导芽分化则相对较难，仅有10％左右的诱导率，老叶片诱导的愈伤无法再分化出芽。
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| 关 键 词： | 榉树 叶器官 愈伤 分化 |
In vitro of Regenerated Plantlets Obtained from Leaf Organ of Zelkova schneideriana |
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| LIU Hal-long^,ZHANG Ri-qing^,WANG Ling-dan^,YANG Kai-tai^,HUANG Jin-shi^,ZENG Yong-ming. In vitro of Regenerated Plantlets Obtained from Leaf Organ of Zelkova schneideriana[J]. , 2013, 0(3): 231-233 |
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| Authors: | LIU Hal-long^ ZHANG Ri-qing^ WANG Ling-dan^ YANG Kai-tai^ HUANG Jin-shi^ ZENG Yong-ming |
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| Affiliation: | 1 ( 1. Guangxi Forestry Research Institute, Key laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Nanning 530002, China; 2. Forestry College, Central South University of Forestry and Technology, Changsha 410004, China) |
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| Abstract: | Leaf organ of Zelkova schneideriana was used as explants to induce callus, then differentiate and regenerate plantlets from callus. Plantlets regeneration technology was studied. Results showed that it was easy to induce callus from leaf organ of Zelkova schneideriana and the inducing ratio of callus could reach above 90% by using young leaves. It was difficult to differentiate and regenerate plantlets by indu cing callus. The ratio of callus differentiation was about 10%. Callus induced form old leaves could not differentiate and regenerate plantlets. |
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| Keywords: | Zelkova schneideriana leaf organ callus differentiation |
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