高效液相色谱柱前衍生化法测定饲料中的盐霉素

以甲醇∶1 5 %醋酸水溶液 (90∶10 ,v/v)为流动相 ,2 ,4 二硝基苯肼 (DNP)为衍生剂 ,应用高效液相色谱柱前衍生化法 ,采用紫外检测方法对饲料中的盐霉素进行了测定。盐霉素标准品的保留时间为 8 99min ,饲料中盐霉素的保留时间为 8 91min。经测定盐霉素标准液的标准曲线回归方程与相关系数 ,其线性关系理想 ,重现性好。采用此方法测得的总回收率为 89 0 6% ,总变异系数为 1 73%。此方法的检测限为 1mg/kg。

Liquid Chromatography Detection of Monensin in Poultry Feeds Using Pre-column Derivatization

An effective analytical procedure of liquid chromatography with ultraviolet detection of monensin in poultry feeds using pre column derivatization was tested. The derivatization mixture, which was derivatized with 2,4 dinitrophenylhydrazine (DNP), was analyzed with methanol-1.5% aqueous acetic acid (90:10, v/v ) as eluent. The RT of standard monensin was 8.99 minutes, and that of monensin in poultry feeds was 8.91 minutes. The data reported showed that the method had good linearity and repeatability results. The total recovery for monensin was 89.06%, and relative standard deviation was 1.73%. The detection limit of the method was 1 mg/kg.