| 成熟猕猴桃果实中乙烯受体基因Ad-ETR2的克隆与序列分析 |
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| 引用本文： | 魏绍冲,陈昆松.成熟猕猴桃果实中乙烯受体基因Ad-ETR2的克隆与序列分析[J].果树学报,2004,21(6):544-547. |
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| 作者姓名： | 魏绍冲 陈昆松 |
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| 作者单位： | 浙江大学果实分子生理与生物技术实验室/农业部园艺植物生长发育与生物技术重点开放实验室,浙江,杭州,310029 |
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| 基金项目： | 国家重点基础研究发展规划项目(973项目)(G2000046806),国家自然科学基金资助项目(30170660),高等学校博士学科点专项科研基金项目资助(20040335022). |
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| 摘 要： | 在分析GenBank登录的植物乙烯受体家族氨基酸和核苷酸保守区序列基础上,设计简并引物,通过RT-PCR扩增出1个657bp大小的cDNA片段,测序结果表明,该片段穴Ad-ETR2雪编码219个氨基酸,它与其它植物乙烯受体核苷酸同源性为79.9%～86.5%,氨基酸的同源性为85.4%～95.9%,序列分析推断该基因片段可能是与番茄Le-ETR1结构和功能类似的一类乙烯受体。
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| 关 键 词： | 猕猴桃 乙烯受体 果实成熟 序列分析 |
| 文章编号： | 1009-9980(2004)06-544-04 |
| 修稿时间： | 2004年7月1日 |
Cloning and Sequence Analysis of An Ethylene Receptor Fragment (Ad-ETR2) From Ripening Kiwifruit |
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| Wei Shaochong,Chen Kunsong.Cloning and Sequence Analysis of An Ethylene Receptor Fragment (Ad-ETR2) From Ripening Kiwifruit[J].Journal of Fruit Science,2004,21(6):544-547. |
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| Authors: | Wei Shaochong Chen Kunsong |
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| Institution: | Wei Shaochong and Chen Kunsong* |
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| Abstract: | A pair of degenerated primers was designed according to the conserved sequences of plant ethylene receptor genes,and a 657 bp fragment was amplified by RT- PCR. The results suggested that this fragment,referring to as Ad-ETR2, encoded 219 amino acids. Ad-ETR2 shared 79.9%～80% and 85.4%～95.9% similarity with the ethylene receptors from other plants at nucleotide and polypeptide level,respectively. This fragment seemed to be a ethylene receptor similar to tomato Le-ETR1 based on sequence analysis. |
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| Keywords: | Kiwifruit Ethylene receptor Fruit ripening Sequence analysis |
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