RFLP analysis of cytoplasmic male-sterile lines of pigeonpea [Cajanus cajan (L.) Millsp.] developed by interspecific crosses |
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Authors: | S Sivaramakrishnan K Seetha A Nageshwar Rao Laxman Singh |
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Institution: | (1) Cellular and Molecular Biology Division, India;(2) Genetic Enhancement Division, International Crops Institute for the Semi-Arid Tropics (ICRISAT), 502 324 Andhra Pradesh, India |
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Abstract: | Total DNA from three putative cytoplasmic male sterile (CMS) progenies derived from crosses between the wild species Cajanus
sericeus and the cultivated species Cajanus cajan, five C. cajan, one accession of C. sericeus and two genetic male sterile
lines of pigeonpea were compared for their RFLP patterns using maize mitochondrial DNA (mtDNA) specific probes. Three putative
cytoplasmic male sterile (CMS) progenies from the multiple cross genome transfer of pigeonpea lines (CMS 7–1, CMS 12–3, and
CMS 33–1) showed hybridization patterns identical to that of C. sericeus when DNA was digested with EcoRI and HindIII and
probed with maize mtDNA clones. The results suggested that these putative CMS progenies have the mitochondria of the female
wild species parent. The hybridization patterns of the three male parental lines used in the development of the CMS progenies
were similar in all the restriction enzyme-probe combinations except HindIII-atp6. The genetic male sterile lines, MS Prabhat
and QMS 1 differed from each other in their hybridization pattern. The genomic DNA hybridization pattern of HindIII digested
DNA from ICPL 87 differed from the other pigeonpea lines when probed with the maize mtDNA clones. The cluster analysis of
the hybridization data suggested the occurrence of variation in the mitochondrial genome even among the cultivated species.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Cajanus sericeus CMS mtDNA RFLP pigeonpea Cajanus cajan |
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