异育银卿口服葡萄糖后血糖、血脂和肝糖原的变化

禁食 4周后对体重为 46± 5g的异育银鲫进行了葡萄糖耐量试验。口服 16 7mg·(10 0g) -1体重的葡萄糖之前及之后的 1、2、3、4、5、6、8、10、14、18和 2 4h分别抽血、取肝胰腺 ,分析血糖、血脂和肝糖原含量。实验结果表明 ,血糖在口服葡萄糖后 3h内迅速升高 ,并于 3h时达最高水平 ,以后逐渐下降。口服葡萄糖后 1h血脂含量最高 ,然后开始下降 ,并于 8h时又开始升高。口服葡萄糖后的最初 2h内肝糖原含量显著降低 ,随后开始提高 ,并在 6h时达最高峰。用体重为 (16 4± 12 )g的异育银鲫为材料禁食 4周后灌喂不同剂量的葡萄糖 ,研究葡萄糖剂量对血糖和肝糖原变化规律的影响。结果表明 ,随着口服葡萄糖剂量的增加 ,血糖升高幅度加大 ,而肝糖原含量则随葡萄糖剂量增加而减少。上述实验结果提示异育银鲫在胰岛素的分泌方面与哺乳类存在本质的不同。

The change of plasma glucose, plasma triglyceride and liver glycogen after oral glucose administration in allogynogenetic silver crucian carp

Glucose to lerance test was performed with allogynogenetic silver crucian carp( Carassius auralus gibelio a @ Cyprinus carpio ` ) , whose initial weight was 46 ? 5g, after the f ish had fasted for 4 weeks. Blood and liver were collected before and 1, 2, 3, 4, 5, 6, 8, 10, 14, 18 and 24h af ter being administered orally with 167mg glucose per 100g body weight . The results showed that the plasma glucose level increased sharply during 3h and reached a peak at 3h after oral administration, and then decreased slowly. Plasma triglyceride peaked at 1h after administration and thereafter started to decrease, which re-increased after 8h. During the first 2h after glucose administration the liver glycogen content decreased significantly, and thereafter increased to a peak value, which was reached 6h after glucose administration. Different doses of glucose were administered to the same species , which had fasted for 4 weeks and whose initial average body weight was 164 ? 12g, to investigate the change of plasma glucose level and liver glycogen content. The results showed that with the dose of glucose increasing , the plasma glucose level increased. However, the liver glycogen content decreased with the increasing of glucose dose. These results indicated that there is native difference in secretion of insulin between allogynogenetic silver crucian carp and mammals.