In vitro mass tuberisation as a contribution to potato micropropagation

Summary Mass tuberisation was obtained on plantlets growing from nodal potato cuttings cultured in vitro on Murashige-Skoog (MS) medium under continuous light of 5000 lux at 24–25°C. Tuber formation was stimulated by transferring the plantlets to MS or White-Nitsch-Morel (WhNM) liquid or solid media supplemented with 8% sucrose, 2 mg 1⁻¹ benzyladenine (BAP), 2 mg 1⁻¹ naphthalenacetic acid (NAA) and/or 100 mg 1⁻¹ (2-chloroethyl) trimethylammonium chloride (CCC). Liquid media invariably induced heavier minitubers (390–790 mg each). The process to tuberisation took only two months. Minitubers always produced normal growing plants after dormancy break.