| 3种微生物诱导的家蚕attacin基因异常表达形式的研究 |
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| 引用本文： | 孔卫青,杨金宏. 3种微生物诱导的家蚕attacin基因异常表达形式的研究[J]. 安徽农业科学, 2009, 37(6): 2407-2408 |
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| 作者姓名： | 孔卫青 杨金宏 |
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| 作者单位： | 安康学院陕西省蚕桑重点实验室,陕西安康,725000;安康学院陕西省蚕桑重点实验室,陕西安康,725000 |
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| 基金项目： | 陕西省教育厅项目，安康学院科研启动专项经费项目 |
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| 摘 要： | [目的]研究家蚕抗菌肽attacin基因在不同诱导源诱导时的表达情况。[方法]应用3种外源微生物BmNPV、JM109以及农杆菌LBA4404对家蚕进行诱导,半定量PCR检测attacin基因在诱导后的家蚕血液和脂肪体的表达情况,对诱导表达产物进行克隆测序及进一步的分析。[结果]attacin基因在所有诱导后的脂肪体中均出现1条800 bp左右的特异表达条带,克隆测序（GenBank登录号：FJ373020）后发现其产生由正常表达形式的2个内含子未被剪接引起,且原序列第1内含子5′端的1个TGA终止密码子使得加长的特异表达序列翻译氨基酸时在此处终止,最终导致了attacin C末端结构的缺失。[结论]attacin基因有2种剪接形式,该研究对探明atta-cin基因在家蚕免疫中的作用具有参考价值。
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| 关 键 词： | 家蚕 attacin 诱导表达 剪接 免疫 |
Abnormal Expression of Silkworm attacin Gene Induced by 3 Kinds of Microorganisms |
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| KONG Wei-qing et al. Abnormal Expression of Silkworm attacin Gene Induced by 3 Kinds of Microorganisms[J]. Journal of Anhui Agricultural Sciences, 2009, 37(6): 2407-2408 |
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| Authors: | KONG Wei-qing et al |
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| Affiliation: | KONG Wei-qing et al(The Key Sericultural Laboratory of Shaanxi,Ankang University,Ankang,Shaanxi 725000) |
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| Abstract: | [Objective] The aim of this research was to study the expression of silkworm attacin gene induced by some different microorganisms.[Method] The silkworms were induced by 3 kinds of exogenous microorganisms,that is,BmNPV,JM109 and Agrobacterium LBA4404.After induction,the expression level in silkworm blood and fat body were detected by semi-quantitative PCR and subsequently the PCR products were cloned and sequenced for further analysis.[Result]A specific band,about 800 bp,appeared in fat body of all induced... |
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| Keywords: | attacin |
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