HrpG regulates type II secretory proteins in Xanthomonas axonopodis pv. citri |
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Authors: | Akihiro Yamazaki Hisae Hirata Shinji Tsuyumu |
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Institution: | (1) Faculty of Agriculture, Shizuoka University (The Gifu United Graduate School of Agricultural Sciences), 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;(2) Department of Biological Science, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;(3) Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan |
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Abstract: | HrpG, a two-component response regulator-like protein, is a key regulator of the type III secretion system (T3SS) in Xanthomonas spp. In X. campestris pv. vesicatoria, HrpG with a single amino acid substitution (HrpG*) gains the ability to induce the expression of T3SS-related genes even
under nutrient-rich conditions. In this study, we investigated the role of HrpG in the synthesis of the secretory protein
using HrpG* in strain NA-1 of X. axonopodis pv. citri (Xac NA-1), a causal agent of citrus canker. Eleven proteins secreted via a type II secretion system (T2SS) were induced
by HrpG*. In proteomic analyses, six of the 11 proteins were identified as extracellular enzymes, and the others as a fimbrial
biogenesis-related protein, a type IV-related protein, two hypothetical proteins, and a conserved hypothetical protein. Further
analysis of these proteins revealed that the genes coding all 11 proteins were upregulated by HrpG*, even though they had
different expression patterns for HrpXct-dependency. The data indicated that HrpG, a key regulator of T3SS, also acts as a
positive regulator of certain proteins secreted via a T2SS in Xac NA-1. |
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Keywords: | Xanthomonas axonopodis pv citri Citrus canker Type II secretion system Type III secretion system hrp Extracellular enzyme |
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