Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal |
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| Authors: | Luís Cardoso Joana Tuna Lisete Vieira Yael Yisaschar-Mekuzas Gad Baneth |
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| Affiliation: | 1. Department of Veterinary Sciences and CECAV, University of Trás-os-Montes e Alto Douro, P.O. Box 1013, 5001-801 Vila Real, Portugal;2. Parasite Disease Group, Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal;3. Os Bichos Veterinary Clinic, 5400-266 Chaves, Portugal;4. School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel;1. Laboratorio de Entomología, Programa Investigación en Medicina Poblacional, Escuela de Medicina Veterinaria, Universidad Nacional, P.O. Box 86-3000 Heredia, Costa Rica;2. Laboratorio de Parasitología, Escuela de Medicina Veterinaria, Universidad Nacional, P.O. Box 86-3000 Heredia, Costa Rica;1. Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Buenos Aires City, Argentina;2. Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain;1. Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte – Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay;2. Facultad de Veterinaria, CENUR Litoral Norte – Salto, Universidad de la República, Rivera 1350, CP 50000, Salto, Uruguay;1. Instituto de Zoonosis Luis Pasteur, Ministerio de Salud, Gobierno de la Ciudad Autónoma de Buenos Aires, Av Díaz Vélez 4821, C1405DCD Caba, Argentina;2. Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, CP 2300 Rafaela, Santa Fe, Argentina;1. Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk 39660, South Korea;2. College of Veterinary Medicine, Kyungpook National University, Daegu 41566, South Korea;3. Cardiovascular Research Institute, Kyungpook National University, Daegu 41944, South Korea |
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| Abstract: | Tick-transmitted rickettsial pathogens belonging to the Ehrlichia and Anaplasma genera can infect dogs and humans. In this study, four dogs from the North of Portugal, in which an ehrlichial disease was suspected clinically, were tested by molecular methods. After DNA extraction from blood on filter paper, a 345 bp fragment of the Ehrlichia/Anaplasma 16S rRNA gene was amplified by the polymerase chain reaction (PCR). Sequence analysis of PCR products revealed one dog infected with Ehrlichia canis and three with Anaplasma platys. One of these latter animals was co-infected with Babesia canis subspecies vogeli. This is the first report of the genetic characterisation of both A. platys and E. canis in naturally infected dogs from the North of Portugal. |
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