| 杨树诱导芽分化中6-BA和NAA配比优化研究 |
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| 引用本文： | 卢小三,范素杰,张帅,何浩,熊东旭,王晓光,李良,涂炳坤.杨树诱导芽分化中6-BA和NAA配比优化研究[J].湖北林业科技,2010(3):17-21. |
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| 作者姓名： | 卢小三 范素杰 张帅 何浩 熊东旭 王晓光 李良 涂炳坤 |
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| 作者单位： | 1. 华中农业大学园艺林学学院,武汉,430070
2. 湖北省林业科学研究院,武汉,430075 |
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| 摘 要： | 以杨树无性系LH05-143的叶柄为外植体,利用二次回归正交试验设计对其再生诱导不定芽中的影响因素6-BA和NAA的浓度进行定量优化,实现对再生系统中芽诱导试验影响因子植物激素浓度的优化,同时扩充了再生系统的外植体资料,为再生体系增加了重要的内容。试验结果表明:诱导培养基中6-BA浓度为1.75mg/L、NAA浓度为0.30mg/L时,叶片分化不定芽数可以达2.68;而6-BA浓度为1.74~2.64mg/L,NAA浓度为0.06~0.73mg/L范围内时,有95%的可靠性产生不定芽的叶片分化不定芽数大于1.5。
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| 关 键 词： | 二次回归正交设计 杨树 叶柄 不定芽诱导 |
Optimize the Concentration of 6-BA and NAA in the Introduction of Adventitious buds |
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| Lu Xiaosan,Fan Sujie,Zhang Shuai,He Hao,Xiong dongxu,Wang Xiaoguang,Li Liang,Tu Bingkun.Optimize the Concentration of 6-BA and NAA in the Introduction of Adventitious buds[J].Hubei Forestry Science and Technology,2010(3):17-21. |
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| Authors: | Lu Xiaosan Fan Sujie Zhang Shuai He Hao Xiong dongxu Wang Xiaoguang Li Liang Tu Bingkun |
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| Institution: | Lu Xiaosan(1) Fan Sujie(1) Zhang Shuai(1) He Hao(1)Xiong dongxu(1) Wang Xiaoguang(2) Li Liang(1) Tu Bingkun(1)(1.Huazhong Agricultural University Wuhan 430070,2.Hubei Academy of Forestry Wuhan 430075) |
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| Abstract: | Optimal hormone concentrations of 6-BA and NAA and their combinations were tested with quadratic regressive orthogonal design for their effect on the introduction for petiole the adventitious buds of Poplar clone LH05-143.Implementation of the regeneration bud induction system tests of plant hormone factor optimization,increase important of explant content for the regeneration system.the results showed that the best combination of the 6-BA and NAA was 1.75 mg/L and 0.30mg/L,and the average number of adventi... |
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| Keywords: | quadratic regressive orthogonal design Poplar petiole adventitious buds |
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